geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM)


, : Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 111(5): 888-897

The low nutritive value of maize endosperm protein is genetically corrected in quality protein maize (QPM), which contains the opaque 2 gene along with numerous modifiers for kernel hardness. We report here a two generation marker-based backcross breeding program for incorporation of the opaque 2 gene along with phenotypic selection for kernel modification in the background of an early maturing normal maize inbred line, V25. Using the flanking marker distances from opaque 2 gene in the cross V 25 xCML 176, optimum population size for the BC(2) generation was computed in such a way that at least one double recombinant could be obtained. Whole genome background selection in the BC(2) generation identified three plants with 93 to 96% recurrent parent genome content. The three BC(2)F(2) families derived from marker identified BC(2) individuals were subjected to foreground selection and phenotypic selection for kernel modification. The tryptophan concentration in endosperm protein was significantly enhanced in all the three classes of kernel modification viz., less than 25%, 25--50% and more than 50% opaqueness. BC(2)F(3) lines developed from the hard endosperm kernels were evaluated for desirable agronomic and biochemical traits in replicated trials and the best line was chosen to represent the QPM version of V25, with tryptophan concentration of 0.85% in protein. The integrated breeding strategy reported here can be applied to reduce genetic drag as well as the time involved in a conventional line conversion program, and would prove valuable in rapid development of specialty corn germ plasm.

US$19.90

PMID: 16034586

DOI: 10.1007/s00122-005-0011-6


Other references

Katulska, K.; Wykrętowicz, M.; Minczykowski, A.; Krauze, T.; Milewska, A.; Piskorski, J.; Marciniak, R.; Stajgis, M.; Wysocki, H.; Guzik, P.; Wykrętowicz, A., 2015: Gray matter volume in relation to cardio-vascular stiffness. Hemodynamic disturbances are associated with aging as well as the chronic process of left ventricular and arterial stiffening. This process can influence gray matter volume and thereby contribute to brain atrophy. We performed a comprehensive asse...

Berner, T.; Evenari, M., 1978: The influence of temperature and light penetration on the abundance of the hypolithic algae in the negev desert of israel. Hypolithic algae were found under flint pebbles in the northern part of the Negev desert in Israel. The algae appeared in the contact area between the stone and the loess soil in which it is partially buried. Two types of flint were found in the r...

Anonymous, 1995: "Mama Condom" preaches safe-sex at a truck-stop. Efforts to stop the spread of AIDS at a small town which provides food, lodging, and sex for truck drivers on the highway from Uganda through Tanzania to Burundi center on using prostitute peer educators. The trained peer educators visit guest hou...

Michaud, H-Alexandre.; Eliaou, J-François.; Lafont, V.; Bonnefoy, N.; Gros, L., 2015: Tumor antigen-targeting monoclonal antibody-based immunotherapy: Orchestrating combined strategies for the development of long-term antitumor immunity. NlmCategory="UNASSIGNED">Tumor antigen (TA)-targeting monoclonal antibody (mAb)-based treatments are considered to be one of the most successful strategies in cancer therapy. Besides targeting TAs and inducing tumor cell death, such a...

Meng, Z.H.; Baker, J.F.; Hudson, A.; Schumacher, H.R.; Baker, D.G., 1996: Crystal-induced tumor necrosis factor and interleukin 6 production by a mononuclear cell line is controlled by different intracellular pathways. Arthritis & Rheumatism 39(9 SUPPL ): S83

Reis, P.; Lourenco, P.; Domingos, A.C.emente, A.; Pais, M.; Malcata, F., 2000: Applicability of extracts from Centaurea calcitrapa in ripening of bovine cheese. Aqueous extracts obtained from cell suspension cultures of Centaurea calcitrapa were used as proteolytic additive in the manufacture of a commercial bovine cheese, coagulated with animal rennet and typically ripened for 28 d. The cheese was assess...

Callahan S.K.; Von Hoff D.D., 1981: Growth of oat cell carcinoma of the lung in the human tumor cloning assay. Stem Cells (Basel) 1(4-5): 295

Niesten, D.; van der Sanden, W.J.M.; Gerritsen, A.E., 2016: The impact of frailty on the oral care behaviour and dental service use of elderly people. Om meer inzicht te krijgen in de manier waarop tandartsbezoek en mondverzorging wordt beïnvloed door de mate van kwetsbaarheid en verschillende kwetsbaarheidfactoren werden 51 kwetsbare ouderen geïnterviewd. Aanvullende informatie werd verzameld...

Krumschnabel, G.; Schwarzbaum, P.J.; Wieser, W., 1999: Energetics of trout hepatocytes during A23187-induced disruption of Ca2+ homeostasis. The impact of an increase of intracellular Ca2+i on the energy metabolism of trout hepatocytes was assessed by applying the Ca2+ ionophore A23187 and studying the consequences of the ensuing elevation of Ca2+i on various metabolic parameters. Afte...

Ho, K.K.; An, J.K., 1980: A collective report on some important diseases and enemies of the honeybee. 1. Varroa jacobsoni. Review.