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Improved discrimination of self-incompatibility S-RNase alleles in cherry and high throughput genotyping by automated sizing of first intron polymerase chain reaction products

, : Improved discrimination of self-incompatibility S-RNase alleles in cherry and high throughput genotyping by automated sizing of first intron polymerase chain reaction products. Plant breeding 125(3): 305-307

A novel polymerase chain reaction (PCR) approach to determine and confirm the self-incompatibility (S) genotype of cherries is reported. The method involves PCR amplification with a new pair of consensus primers that immediately flank the first intron of cherry S-RNases, one of which is fluorescently labelled. Fluorescent amplification products range from 234 to c. 460 bp and can be sized accurately on an automated sequencer. Thirteen S alleles reported in sweet cherry can be distinguished, except for S[subscript 2] and S[subscript 7], which have an amplification product of exactly the same size. S[subscript 13], which is also amplified, gives a microsatellite-like trace which shows minor intra-allelic length variation. This method gives fast and accurate results and should be especially useful for medium/high-throughput genotyping of wild and cultivated cherries.


DOI: 10.1111/j.1439-0523.2006.01205.x

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