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A rapid method for evaluating the fertilizing capacity of frozen thawed sperm of poultry

, : A rapid method for evaluating the fertilizing capacity of frozen thawed sperm of poultry. Sel'skokhozyaistvennaya Biologiya (12): 77-80

A fluorometric express-method of evaluation of cock's sperm presented in the paper is based on the use of a two dye mixture: thyasine red and ethidium bromide, as fluorochrome. It permits simultaneous detection of injuries of acrosome and spermatozoid head. It takes not more than 5-10 min to evaluate the fertilizing capacity of sperm specimen. The possibility is being considered to use the given method for spermatozoid evalution for the development of freezing-defrosting regimens, sperm dilution media, in studies on cryoprotector toxicity, and development of regimens of male use in clinical practice.


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