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Alpha amylase and beta amylase homoeoloci in species related to wheat


, : Alpha amylase and beta amylase homoeoloci in species related to wheat. Genetical Research 49(2): 93-104

A study of .alpha.-amylase isozyme patterns from gibberellin-induced endosperms of wheat-alien genotypes (amphiploid, addition and substitution lines) resolved by flat-bed isoelectric focusing identified homoeoloci for .alpha.-Amy-1 (malt .alpha.-AMY-1 genes) on chromosomes 6H of Hordeum vulgare, 6RL of Secale cereale, 6Rm of S. montanum and 6E of Agropyron elongatum. Homoeoloci for .alpha.-Amy-2 (green .alpha.-AMY-2 genes) were identified on chromosomes 7HchL of Hordeum chilense, 7RL of Secale cereale, 7Sb of Aegilops bicornis, 7U of Ae. umbellulata and 7EL of Agropyron elongatum. Analysis of mature grain .beta.-amylase identified .beta.-Amy-1 loci on chromosomes 4H of H. vulgare, 4Hch of H. chilense, 4S1 of Ae. sharonensis and Ae. longissima and .beta.-Amy-2 loci on chromosomes 5RL of S. cereale and 5U of Ae. umbellulata. These gene locations provide further evidence for the homoeology of the alien chromosomes with wheat and for the conservation of gene synteny among wheat and its relatives.

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