+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Amino Acid Metabolism of Lemna minor L. : III. Responses to Aminooxyacetate

, : Amino Acid Metabolism of Lemna minor L. : III. Responses to Aminooxyacetate. Plant Physiology 87(2): 447-453

Aminooxyacetate, a known inhibitor of transaminase reactions and glycine decarboxylase, promotes rapid depletion of the free pools of serine and aspartate in nitrate grown Lemna minor L. This compound markedly inhibits the methionine sulfoximine-induced accumulation of free ammonium ions and greatly restricts the methionine sulfoximine-induced depletion of amino acids such as glutamate, alanine, and asparagine. These results suggest that glutamate, alanine, and asparagine are normally catabolized to ammonia by transaminase-dependent pathways rather than via dehydrogenase or amidohydrolase reactions. Aminooxyacetate does not inhibit the methionine sulfoximine-induced irreversible deactivation of glutamine synthetase in vivo, indicating that these effects cannot be simply ascribed to inhibition of methionine sulfoximine uptake by aminooxyacetate. This transaminase inhibitor promotes extensive accumulation of several amino acids including valine, leucine, isoleucine, alanine, glycine, threonine, proline, phenylalanine, lysine, and tyrosine. Since the aminooxyacetate induced accumulations of valine, leucine, and isoleucine are not inhibited by the branched-chain amino acid biosynthesis inhibitor, chlorsulfuron, these amino acid accumulations most probably involve protein turnover. Depletions of soluble protein bound amino acids are shown to be approximately stoichiometric with the free amino acid pool accumulations induced by aminooxyacetate. Aminooxyacetate is demonstrated to inhibit the chlorsulfuron-induced accumulation of .alpha.-amino-n-butyrate in L. minor, supporting the notion that this amino acid is derived from transamination of 2-oxobutyrate.


PMID: 16666162

DOI: 10.1104/pp.87.2.447

Other references

Petersen, K.F., 1971: Serodiagnosis of rheumatic fever and acute glomerulonephritis. Medizinische Klinik 66(14): 503-505

Lehmann, P., 1977: Paid blood: the hepatitis connection. Fda Consumer 11(9): 8-11

Hubschle, O.J.B.; Tjipura Zaire, G.; Abusugra, I.; Francesca, G. di; Mettler, F.; Pini, A.; Morein, B., 2003: Experimental field trial with an immunostimulating complex (ISCOM) vaccine against contagious bovine pleuropneumonia. The experimental field trial with an immunostimulating complex (ISCOM) vaccine has been an occasion to explore the role of a Th1 response in the pathogenesis caused by Mycoplasma mycoides subsp. mycoides small colony (MmmSC) and in immune protecti...

Wainberg M.A., 1984: Partial reversal of in vitro immuno suppression in acquired immune deficiency syndrome. Journal of Cellular Biochemistry Supplement (8 PART A): 17

Vollmer, C.M., 2013: The economics of pancreas surgery. Pancreas surgery is a paradigm for high-acuity surgical specialization. Given the current intrigue over containing health care expenditures, pancreas surgery provides an ideal model to investigate the cost of care. This article explores the econom...

Foley, J.F.; Lemon, H.M.; Miller, D.M.; Kessinger, A., 1972: The treatment of metastatic testicular tumors. Journal of Urology 108(3): 439-442

Coutu, B.G.; Wilke, C.T.; Yuan, J.; Cao, Q.; Vernon, M.R.; Lee, C.; Bachanova, V.; Dusenbery, K.E., 2017: Consolidative Radiotherapy After Autologous Stem Cell Transplantation for Relapsed or Refractory Diffuse Large B-cell Lymphoma. We evaluated the role of consolidative radiotherapy (RT) for patients undergoing high-dose chemotherapy (HDC) and autologous stem cell transplantation (ASCT) for relapsed or refractory diffuse large B-cell lymphoma (DLBCL). We reviewed the medical...

Ieiri T., 1973: Possible multiplex control of synthesis and release of rat anterior pituitary hormones. Proceedings Of Annual Meeting Of Japan Endocrinological Society: 2

Menkhorst, Pw, 1975: Ground parrot habitat at Sand-Patch Point, Victoria. Australian Bird Watcher2: 41-44

Schoenfield, L.J.; Marks, J.W., 1993: Oral and contact dissolution of gallstones. The appropriate selection of patients for treatment with oral ursodeoxycholic acid (UDCA)--a drug that has virtually no side effects--results in about 50% of patients experiencing safe dissolution of gallstones within 2 years. Eligible patients ha...