geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An evaluation of the conditions necessary for optimal protein a gold labeling of capsular antigen in ultra thin methacrylate sections of the bacterium pasteurella haemolytica


, : An evaluation of the conditions necessary for optimal protein a gold labeling of capsular antigen in ultra thin methacrylate sections of the bacterium pasteurella haemolytica. Histochemical Journal 16(2): 151-164

The protein A-Au (PAG) probe is a particulate immunocytochemical probe that is eminently suitable for quantification. The effect of probe concentration, variation of labeling on different sections within a single grid, washing procedures, variation of labeling with time and temperature and different storage conditions on the probe were investigated using PAG labeling of capsular antigen on ultrathin methacrylate sections of the bacterium P. haemolytica. In this antigen-antibody system, and using a 20 nm probe, optimal results were achieved with 2 .times. 1012 particles/ml, a labeling time of 60 min at room temperature; the PAG probe, stored at 4.degree. C, should be 1-5 wk old. The efficiency of the probe tested by evaluating different primary antibody concentrations, by evaluating cross reactions of the primary antibody and by evaluating the relative amounts of antibody against internal components of the bacterium present in different antisera.

US$29.90

PMID: 6698803


Other references

Richards, J.M.; Sudowe, R., 2016: Separation of Americium in High Oxidation States from Curium Utilizing Sodium Bismuthate. A simple separation of americium from curium would support closure of the nuclear fuel cycle, assist in nuclear forensic analysis, and allow for more accurate measurement of neutron capture properties of (241)Am. Methods for the separation of amer...

Sharping, J.; Okawachi, Y.; Gaeta, A., 2005: Wide bandwidth slow light using a Raman fiber amplifier. We demonstrate an all-optical tunable pulse delay scheme that utilizes the power-dependent variation of the refractive index that accompanies stimulated Raman scattering in an optical fiber. Using this technique, we delay 430-fs pulses by up to 85...

Sonin, B.V., 1968: Obzor literatury,. This review with 44 references indicates that most of the nitrofuran drugs, with the exception of furazolidon, are rapidly and completely absorbed from the gastrointestinal tract. However, the question of the absorption of nitrofurans requires fur...

Oddera, S.; Silvestri, M.; Sacco, O.; Eftimiadi, C.; Rossi, G.A., 1993: Effect of pidotimod on phagocytosis and intracellular killing of Staphylococcus aureus by human circulating polymorphonuclear neutrophils and alveolar macrophages. Drugs Under Experimental and Clinical Research 19 Suppl: 27-35

Laten, H.M.; Morris, R.O., 1993: SIRE-1, a long interspersed repetitive DNA element from soybean with weak sequence similarity to retrotransposons: initial characterization and partial sequence. Gm776 is a 776-bp subregion of a member of an interspersed family of relatively homogeneous repetitive DNA elements from soybean (Glycine max). The fragment was originally amplified from soybean DNA by the polymerase chain reaction using a single...

Demaro, J.A.; Arends, J.J.A.; Jacquin, M.F., 1995: Ultrastructure and amino acid immunoreactivity of projection vs non-projection neurons in trigeminal nucleus principalis. Society for Neuroscience Abstracts 21(1-3): 106

Berry R.F., 1995: Palaeozoic tectonics of western Tasmania. Abstracts - Geological Society of Australia 40: 11-12

Baldwin, Bruce G., 2003: Natural history of the continental tarweeds and the Hawaiian silversword alliance. Carlquist, Sherwin [Editor, Reprint Author], Baldwin, Bruce G [Editor], Carr, Gerald D [Editor] Tarweeds & silverswords: Evolution of the Madiinae (Asteraceae) : 1-16

Woynarowska, B.; Robinson, S.C., 1998: Inhibition of prostate tumor cell adhesion by targeting prostate specific membrane antigen. Proceedings of the American Association for Cancer Research Annual Meeting 39: 200, March

Campbell, M.E.; Costeloe, K.L., 1999: Measurement of intramucosal pH in very low birthweight infants. Pediatric Research 45(4 PART 2): 188A, April