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An investigation of carbon 13 d xylose metabolism in pichia stipitis under aerobic and anaerobic conditions


, : An investigation of carbon 13 d xylose metabolism in pichia stipitis under aerobic and anaerobic conditions. Applied Microbiology & Biotechnology 28(3): 293-296

The uptake of D-[1-13C]xylose, the accumulation of intermediates and the distribution of the label in ethanol in Pichia stipitis under aerobic and anerobic conditions were investigated by nuclear magnetic resonance spectroscopy. The rate-limiting step of D-xylose metabolism under aerobic conditions appeared to be uptake, whereas under anaerobic conditions it was the conversion of xylitol to xylulose. The yeast showed no preference to either the alpha- or beta-forms of D-xylose. Under anaerobic conditions only [2-13C] ethanol was detected and this suggests that NADH but not NADPH was used as cofactor in the conversion of xylose to xylitol. D-Xylose is most likely metabolised by the pentose phosphate pathway in this yeast.

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