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Analysis of 2 carbon to 6 carbon mono carboxylic acids in aqueous solution using gas chromatography


, : Analysis of 2 carbon to 6 carbon mono carboxylic acids in aqueous solution using gas chromatography. Journal of Chromatography 244(2): 337-346

The determination of short chain fatty acids is a key requirement in many fermentations and was studied by gas chromatography. A simple acidification of the fermenter broth with oxalic acid allows direct injection on untreated porous polymer columns with virtually no ghosting problems. The method is suitable for the routine quantification of normal- and iso-monocarboxylic acids with an analysis time of 40 min. For triplicate analyses, the 95% probability confidence limits are .+-. 0.05 for a mean of 1 g/l.

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