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Application of a subcellular membrane antibody binding assay for the analysis of antigen expression in human tumors

, : Application of a subcellular membrane antibody binding assay for the analysis of antigen expression in human tumors. Journal of Cancer Research & Clinical Oncology 111(2): 169-172

A micro-radioisotopic antiglobulin assay has been employed to determine the binding of monoclonal antibodies to subcellular membranes adsorbed to the wells of Terasaki Microtest Plates. A panel of 15 monoclonal antibodies was tested against 22 membrane preparations isolated from normal and malignant colo-rectal, breast and lung tissues. The assay provided an objective evaluation of antibody reactivity permitting the expression of major antigens in samples from individual surgical specimens to be defined.


PMID: 3700463

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