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Artificial capillary perfusion cell culture metabolic studies


, : Artificial capillary perfusion cell culture metabolic studies. In Vitro (Rockville) 14(5): 443-450

Glucose, lactic-acid and O2 metabolism of baby hamster kidney BHK and mouse L929 cells on artificial capillary perfusion units were studied using several different modes of perfusion. After 7-10 days, cells planted in the extracapillary compartment of culture units containing 80-150 fibers reached populations that used 0.073 .+-. 0.025 .mu.mol/min glucose and 0.76 .+-. 0.26 .mu.l/min O2 and excreted 0.078 .+-. 0.038 .mu.mol/min lactic acid. From these data it is estimated that these units contain approximately 2 .times. 107 cells. The metabolic rate of cultures perfused through the capillaries or through the extracapillary compartment was not affected significantly by change in flow rate except at perfusion flow rates .ltoreq. 0.05 ml/min. The cell population, as measured by metabolic activity, did not increase significantly when the serum content of the medium was .ltoreq. 1%. No major differences were found in glucose utilization rates of equal numbers of cells on artificial capillaries, on short-term suspension culture or as monolayers in plastic flasks. Artificial capillary perfusion may provide a simple system for studying metabolism of mammalian cells in culture.

US$19.90

PMID: 352916

DOI: 10.1007/bf02616106


Other references

Poe, W.D., 1950: The effect of endogenous insulin on gastric function; a clinical study. Annals of Internal Medicine 32(2): 270-283

Printz, C., 2014: Cancer and the Affordable Care Act: despite some challenges, most cancer experts say the new law benefits patients and survivors. Cancer 120(13): 1911-1912

Uyemoto, Jk, 1983: Corn lethal necrosis: disease symptoms, control, and epidemiology considerations. Proceedings: International Maize Virus Disease Colloquium and Workshop Wooster Ohio August 2-6-1982 edited by DT Gordon et al: 143

Millan,G.; Agosto,F.; Vazquez,M.; Botto,L.; Lombardi,L.; Juan,L., 2008: Use of clinoptilolite as a carrier for nitrogen fertilizers in soils of the Pampean regions of Argentina. The properties of clinoptilolite related to cationic exchange and ionic selectivity have motivated us to study its application for agronomic purposes. The high potential of the clinoptilolite has been demonstrated as a vehicle for nitrogen fertili...

Mitra, S.; Nath, D.; Majumder, G.C., 1994: Purification and characterization of a protein kinase from goat sperm plasma membrane. A protein kinase that causes phosphorylation of serine and threonine residues of casein has been partially purified from goat cauda-epididymal sperm plasma membrane and characterized. The kinase, solubilized from the membrane with 1.0% Triton X-10...

Keller, W., 1975: Determination of the number of super helical turns in sv 40 dna by gel electrophoresis. Proceedings of the National Academy of Sciences of the United States of America 72(12): 4876-4880

Sierra, J.J., 1971: Practical suggestions in endodontics. Revista Guatemalteca de Estomatologia 1(2): 69-70

Underwood, E.J., 1977: Trace elements in human and animal nutrition. This book details the physiological roles of the trace elements in human and animal nutrition, requirements and tolerances of the elements, their interactions with each other and with other nutrients and compounds, and the biochemical and patholog...

Aurousseau, M.H.; Samama, M.M.; Belhassen, A.; Herve, F.; Hugues, J.N., 1995: Risk of thromboembolism in relation to an in-vitro fertilization programme: three case reports. Severe thrombotic events following ovarian stimulation for in-vitro fertilization (IVF) procedures in three women are reported. None of these patients presented any concomitant clinical sign of ovarian hyperstimulation syndrome. Coagulation inhibi...

Allan, C.A., 1989: Characteristics and help-seeking patterns of attenders at a community-based voluntary agency and an alcohol and drug treatment unit. The assumption that clients using community-based facilities in the form of Councils on Alcohol have less serious alcohol problems uncomplicated by the physical, social and psychological difficulties found in those attending Alcohol Treatment Unit...