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Artificial capillary perfusion cell culture metabolic studies

, : Artificial capillary perfusion cell culture metabolic studies. In Vitro (Rockville) 14(5): 443-450

Glucose, lactic-acid and O2 metabolism of baby hamster kidney BHK and mouse L929 cells on artificial capillary perfusion units were studied using several different modes of perfusion. After 7-10 days, cells planted in the extracapillary compartment of culture units containing 80-150 fibers reached populations that used 0.073 .+-. 0.025 .mu.mol/min glucose and 0.76 .+-. 0.26 .mu.l/min O2 and excreted 0.078 .+-. 0.038 .mu.mol/min lactic acid. From these data it is estimated that these units contain approximately 2 .times. 107 cells. The metabolic rate of cultures perfused through the capillaries or through the extracapillary compartment was not affected significantly by change in flow rate except at perfusion flow rates .ltoreq. 0.05 ml/min. The cell population, as measured by metabolic activity, did not increase significantly when the serum content of the medium was .ltoreq. 1%. No major differences were found in glucose utilization rates of equal numbers of cells on artificial capillaries, on short-term suspension culture or as monolayers in plastic flasks. Artificial capillary perfusion may provide a simple system for studying metabolism of mammalian cells in culture.


PMID: 352916

DOI: 10.1007/bf02616106

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