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Ascaris suum immune response in the guinea pig part 1 lymphoid cell responses during primary infections

, : Ascaris suum immune response in the guinea pig part 1 lymphoid cell responses during primary infections. Experimental Parasitology 41(1): 141-159

The lymphoid cell responses of the mesenteric, hepatic and mediastinal lymph nodes of the spleen and of the Peyer's patches of normal guinea pigs that were orally infected with 10,000 infective eggs of A. suum (group I), received 10,000 artificially hatched 2nd-stage larvae of A. suum via the mesenteric vein (group II), or received 1500 3rd-stage larvae of A. suum via the saphenous vein (group III) were assessed by antigen-induced lymphocyte-transformation, rosette-formation and rosette-plaguing techniques. Antigen-induced lymphocyte transformation measured qualitatively the antigen-sensitive cell population, rosette formation measured quantitatively the immunoglobulin[Ig]-receptored cell population and rosette-plaquing measured quantitatively the receptored antibody-producing and the nonreceptored antibody-producing cell populations. Antigen-induced lymphocyte-transformation and rosette-formation studies showed that the immune response to A. suum was local in character at least for the first 11-12 days of infection as exemplified by the marked responses of the draining lymph nodes at the time the parasite was migrating through the tissues of the host. Rosette-plaquing studies in conjunction with the rosette-inhibition studies showed the the lymphoid cell responses of the draining lymph nodes progressed from an antigen-sensitive state to an immunoglobulin-receptored state, then to a receptored state capable of antibody production and finally to a nonreceptored antibody-producing state. In animals of group I, the lymphoid cells of the mesenteric, hepatic and mediastinal lymph nodes formed predominantly IgM and IgE antigen-specific rosettes (peak responses at days 2, 5 and 7 for the mesenteric, hepatic and mediastinal nodes, respectively, rosette-plaques (peak responses at days 5, 7 and 9 for the mesenteric, hepatic, and mediastinal nodes, respectively), and plaques (peak responses at days 7, 9 and 12 for the mesenteric, hepatic and mediastinal nodes, respectively). The IgE-responsive cells were appreciably higher in the mediastinal lymph nodes than in the other lymphoid organs studied. The responses of the spleen were predominantly IgM in character and they increased gradually as the infection progressed without showing detectable peak responses. The lymphocytes of the Peyer's patches did not exhibit antigen-specific rosetting, rosette-plaquing, or plaquing responses. In group II animals (elimination of the bowel phase of the infection) only the hepatic and mediastinal nodes exhibited peak responses and these were similar to those of group I animals in the type and pattern of the responses and in the time of their sequential appearance. In animals of group III (elimination of both the bowel and the hepatic phase of the infection) only the mediastinal nodes exhibited peak responses and these were similar to those of groups I and II in the type (predominantly IgM and IgE) and their sequential pattern but differed in the time of their appearance. Peak antigen-specific rosettes, rosette-plaques and plaques occurred in the mediastinal nodes of group III animals at days 2, 5 and 7 after infection, respectively. In each of the responsive lymphoid organs studied, there occurred a certain population of lymphoid cells which was producing immunoglobulins that were not necessarily specific for the A. suum antigen.


Other references

da Motta, AJ., 1996: Roma Locuta Sententia confutata. Hawaiian Shell News. March-April; 443-4: 10-11 New Series 434

Echevarria, R.; Ackerman, L.V., 1967: Spindle and epitheloid cell nevi in the adult. Clinicopathologic report of 26 cases. Cancer 20(2): 175-189

von KOBYLETZKI.S.HMIDT.G., 1963: Experimental studies on the problem of intrauterine asphyxia after labor induction with quinine. Zeitschrift für Geburtshilfe und Gynakologie 160: 200-208

Ondrejickova, A., 1972: Contribution a l'etude de la variabilite de Chlamys (G.) palmata Lamarck. Geologicke Pr Spravy, 59: 203-208

Dyer, T.R.gauskas, A., 2002: Developments in bleaching technology focus on reducing capital, operating costs. Pulp and paper 76(3): 49

Sheng Mao-Ling; Rui-Xing, Z., 2012: Species of ichneumonidae hymenoptera parasitizing orgyia ericae with description of a new species. Four species of Ichneumonidae, Eutanyacra picta (Schrank), Itoplectis viduata (Gravenhorst), Pimpla disparis Viereck, and a new species, Dichrogaster orgyiae Sheng, sp. nov. (Figs 1-7), reared from Orgyia ericae Germar in Ningxia and Inner Mongoli...

Sohn, Y-H.; Cha, C-H.; Oh, H-B.; Kim, M.H.; Choi, S-E.; Kwon, O-J., 2010: MICA polymorphisms and haplotypes with HLA-B and HLA-DRB1 in Koreans. Major histocompatibility complex (MHC) class I chain-related gene A (<i>MICA</i>) is located within the human MHC, centromeric to <i>HLA-B</i> and telomeric to <i>HLA-DRB1</i>. The location of <i>MICA</...

Lundin, O.; Rydgren, B.; Sward, L.; Karlsson, J., 1998: Analgesic effects of intra-articular morphine during and after knee arthroscopy: A comparison of two methods. The objective of this study was to compare the analgesic effects of intro-articularly administrated bupivacaine with bupivacaine/morphine during and after therapeutic knee arthroscopy. In a prospective randomized study, 50 patients with clinical s...

Lechago, S.A.; Carr, J.E.; Kisamore, A.N.; Grow, L.L., 2015: The Effects of Multiple Exemplar Instruction on the Relation Between Listener and Intraverbal Categorization Repertoires. We evaluated the effects of multiple exemplar instruction (MEI) on the relation between listener and intraverbal categorization repertoires of six typically developing preschool-age children using a nonconcurrent multiple-probe design across parti...

Llewellyn-Jones, D., 1959: Chlorothiazide in the treatment of oedema in pregnancy. Medical Journal of Malaya 14: 12-16