geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Asparagusate dehydrogenases and lipoyl dehydrogenase from asparagus mitochondria. Physical, chemical, and enzymatic properties


, : Asparagusate dehydrogenases and lipoyl dehydrogenase from asparagus mitochondria. Physical, chemical, and enzymatic properties. Journal of Biological Chemistry 251(12): 3637-3644

Asparagusate dehydrogenases I and II and lipoyl dehydrogenase were obtained in homogeneous state from asparagus [Asparagus officinalis] mitochondria. They are flavin enzymes with 1 mol of FAD/mol of protein. Asparagusate dehydrogenases I and II and lipoyl dehydrogenase have s20,w of 6.22 S, 6.39 S, and 5.91 S, respectively, and MW of 111,000, 110,000, and 95,000 (sedimentation equilibrium) or 112,000, 112,000 and 92,000 (gel filtration). They are slightly acidic proteins with isoelectric points of 6.75, 5.75, and 6.80. Both asparagusate dehydrogenases catalyzed the reaction Asg(SH)2 [dihydroasparagusic acid] + NAD+ .dblarw. AsgS2 [asparagusic acid] + NADH + H+ and exhibit lipoyl dehydrogenase and diaphorase activities. Lipoyl dehydrogenase is specific for lipoate and has no asparagusate dehydrogenase activity. NADP cannot replace NAD in any case. Optimum pH for substrate reduction of the 3 enzymes are near 5.9. Asparagusate dehydrogenases I and II have Km values of 21.5 mM and 20.0 mM for asparagusate and 3.0 mM and 3.3 mM for lipoate, respectively. Lipoyl dehydrogenase activity of asparagusate dehydrogenases is enhanced by NAD and surfactants such as lecithin and Tween 80, but asparagusate dehydrogenase activity is not enhanced. Asparagusate dehydrogenases are strongly inhibited by mercuric ion, p-chloromercuribenzoic acid, and N-ethylmaleimide. The amino acid composition of the 3 enzymes is presented and discussed.

US$29.90

PMID: 180003


Other references

Ito, M.; Nawa, T.; Mikamo, H.; Tamaya, T., 1998: Lower segment uterine rupture related to early pregnancy by in vitro fertilization and embryo transfer after a previous cesarean delivery. A 31-year old woman with secondary infertility after a previous Cesarean delivery underwent in vitro fertilization and embryo transfer (IVF-ET). She was pregnant by IVF-ET. In the first trimester, however, she began to have genital bleeding and lo...

Guruvayoorappan, C.; Kuttan, G., 2007: Methanol extract of Biophytum sensitivum alters the cytokine profile and inhibits iNOS and COX-2 expression in LPS/Con A stimulated macrophages. Biophytum sensitivum has been used in traditional folk medicine to treat numerous diseases. The molecular mechanism of B. sensitivum pharmacological and biochemical actions of macrophages in inflammation has not been clearly elucidated. We examine...

Miloudi Hlaïbi; Mustapha Hor; Mohamed Riri; Abdelkhalek Benjjar; Jean-François Verchère, 2009: Multinuclear 183W and 13C NMR and Indirect photometry study for the identification and the characterization of new complexes of sugar acids. Colourless tungstate complexes of the sugar acids, l-manonnic, d-glucaric and galactaric acids have been studied in aqueous solutions. Multinuclear 13C and 183W NMR have been used to identify the structures of the complexes and the sites of chelat...

Zubareva, T.V.; Kamenetskaia, T.Iu., 1970: Discontinuing pilocarpine therapy in patients with initial primary glaucoma. Oftalmologicheskii Zhurnal 25(6): 442-444

Martins, I.-Salas; Coelho, L.-Teixeira; Mazzilli, R.-Nilda; Singer, J.-Da-Motta; Souza, C.-Unglert-De; Antonieto-Junior, A.-Eduardo; Pasini, U.; Alonso-Nieto, R.; Alvares, E.-Donizetti; Okani, E.-Tieko, 1993: Atherosclerotic cardiovascular disease, lipemic disorders, hypertension obesity and diabetes mellitus in the population of a metropolitan area of Southeastern Brazil: I. Research methodology. An epidemiological study was carried out into the risk factors for the following atherosclerotic cardiovascular diseases: lipemics disorders, obesity, hypertension, diabetes mellitus as related to some factors which characterize life-style (sedent...

Choi, S.T., 1983: Effects of low temperature and hot water treatment of dormant bulbs on leaf emergence in bulblets during propagation of Easter lily (Lilium longiflorum Thunb.) from scales. In studies with the cultivars Georgia and Hinomoto, low temperature treatment (40 days at 5 degrees C) inhibited leaf emergence in both cultivars. Leaf emergence was not promoted by hot water treatment (1 h at 45 degrees ). The effects of treatmen...

Christoff, A., 1930: The Pleospora disease of cultivated Poppy. This is a detailed account of what is stated to be the most destructive disease of the opium poppy (Papaver somniferum) in Bulgaria, where the plant is extensively cultivated not so much for the sake of the drug as for that of the oil obtained fro...

Staib, C.; Drexler, I.; Sutter, G., 2004: Construction and isolation of recombinant MVA. Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its...

Tomovic, S.; Esmaeili, A.; Chan, N.J.; Shukla, P.A.; Choudhry, O.J.; Liu, J.K.; Eloy, J.Anderson., 2014: High-resolution computed tomography analysis of variations of the sphenoid sinus. Purpose The sphenoid sinus is a complex structure with key variations that are important for endoscopic parasellar approaches. In this study, high-resolution computed tomography (HRCT) scans were analyzed for the frequency of these variations. M...

Park, J.Geun.; Lee, D-Hee.; Moon, Y.Soo.; Kim, K-Hong., 2014: Reversine increases the plasticity of lineage-committed preadipocytes to osteogenesis by inhibiting adipogenesis through induction of TGF-β pathway in vitro. Reversine has been reported to reverse differentiation of lineage-committed cells to mesenchymal stem cells (MSCs), which then enables them to be differentiated into other various lineages. Both adipocytes and osteoblasts are known to originate fr...