geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Bacitracin an inhibitor of the insulin degrading activity of glutathione insulin transhydrogenase


, : Bacitracin an inhibitor of the insulin degrading activity of glutathione insulin transhydrogenase. Biochemical & Biophysical Research Communications 98(2): 431-438

The antibiotic bacitracin, a known inhibitor of insulin degradation by both isolated cells and subcellular organelles, inhibited the ability of purified glutathione-insulin transhydrogenase to split insulin into its constituent A and B chains. This inhibition was demonstated by measuring the formation of insulin degradative products that were both soluble in 5% trichloroacetic acid and chromatographed as the separate chains of insulin on Sephadex G-50. At concentrations of 90 and 300 .mu.M, bacitracin inhibited 50 and 90%, respectively, of the degrading activity of the purified enzyme. Similarly, degradation by crude rat liver lysates was inhibited 50 and 90% by 70 and 250 .mu.M bacitracin, respectively. Kinetic studies indicated that this inhibition was by a complex mechanism that decreased both the Vmax and affinity of the enzyme for insulin. These data raise the possibility that the inhibition of glutathione-insulin transhydrogenase by bacitracin could account for part or all of the effects of this antibiotic on inhibition of insulin degradation by target cells.

US$29.90

PMID: 7013763


Other references

Kuz'min, O.B.; Mikhaĭlenko, P.V., 1987: Role of kidney prostaglandins, kinins and dopamine receptors in the natriuretic effect of ethacrynic acid. It was found in experiments on anesthetized rats that ethacrynic acid (3 mg/kg intravenously) exerted no effect on the blood flow in the internal zone of the cortical layer but significantly increased the blood supply to the median zone of this re...

Weiss, H.P., 1999: National report calls for more teeth in implementing enforcement remedies. Balance 3(1): 4-5

Sawers, R.Gary., 2016: Dormancy: Illuminating How a Microbial Sleeping Beauty Awakens. NlmCategory="UNASSIGNED">Nitrogen limitation induces dormancy in the cyanobacterium Synechocystis. During dormancy, the bacterium neither photosynthesizes nor divides, yet it maintains low-level metabolic activity. A new study shows h...

Simonoff, J.S.; Hochberg, Y.; Reiser, B., 1986: Alternative estimation procedures for Pr(X<Y) in categorized data. Biometrics 42: 5-907

Ringe F., 1971: On the knowledge of the inflorescence of begonia semperflorens var gracilis. Beitraege zur Biologie der Pflanzen 47(3): 437-451

Molina, C.; Mignot, P.; Janin, A.; Kantelip, B.; Delage, J., 1986: Value and limits of bronchial biopsy in asthmatics. Bulletin de L'academie Nationale de Medecine 170(2): 223-229

Bagayoko, F.Y.nkeu, , S.E.bers, J.G.esen, N.V.n-De, 2007: Energy partitioning over the West African savanna: Multi-year evaporation and surface conductance measurements in Eastern Burkina Faso. Seasonal variability of the energy partitioning was analyzed with a combination of eddy fluxes of sensible and latent heat and weather data on intensely farmed land in the savanna area of Eastern Burkina Faso, West Africa. The analysis covers two...

Garver, K.A.; LaPatra, S.E.; Kurath, G., 2005: Efficacy of an infectious hematopoietic necrosis (IHN) virus DNA vaccine in Chinook Oncorhynchus tshawytscha and sockeye O. nerka salmon. The level of protective immunity was determined for Chinook Oncorhynchus tshawytscha and sockeye/kokanee salmon (anadromous and landlocked) O. nerka following intramuscular vaccination with a DNA vaccine against the aquatic rhabdovirus, infectious...

Zaki, M.S.; Burke, J.F.; Trelstad, R.L., 1978: Protective effects of adenosine triphosphate administration in burns. The effects of intramuscular adenosine triphosphate (ATP) administration after thermal skin injury on the ATP content and morphology of the liver and heart were studied in male Sprague-Dawley rats. The results showed that exogenous ATP injected du...

Cope, J.Lack.; Chung, E.; Ohgami, Y.; Quock, R.M., 2010: Antagonism of the antinociceptive effect of nitrous oxide by inhibition of enzyme activity or expression of neuronal nitric oxide synthase in the mouse brain and spinal cord. Previous studies have implicated nitric oxide (NO) in the antinociceptive response to the anesthetic gas nitrous oxide (N(2)O). The present study was conducted to confirm this NO involvement using pharmacological and gene knockdown and knockout st...