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Binding of immunoglobulin g from patients with autoimmune thyroid diseases to solubilized guinea pig fat cell membranes crosslinked with iodine 125 tsh


, : Binding of immunoglobulin g from patients with autoimmune thyroid diseases to solubilized guinea pig fat cell membranes crosslinked with iodine 125 tsh. Endocrinologia Japonica 33(3): 333-344

Binding of immunoglobulin G (IgG) to Triton-solubilized fat cell membranes crosslinked with 125I-TSH was studied by an indirect immunoprecipitation method. Guinea pig fat cell membranes (FCM) contained TSH receptors with an association constant of 1.92 .times. 109 M-1 were first reacted with 125I-TSH, then treated with a crosslinker, dissuccinimidyl suberate. The dissociation of 125I-TSH from the crosslinked 125I-TSH-FCM complexes due to the addition of 100 mU/ml unlabeled TSH was 9.0%, while it was 33% without the treatment. To the Triton-solubilized FCM crosslinked with 125I-TSH, 50 .mu.g each of IgG from 20 normal controls, 20 patients with Graves' disease and 20 with Hashimoto's disease was added and precipitation was effected by adding anti-human IgG. In patients with Graves' disease, 125I-TSH-FCM complexes immunoprecipitated ranged from 1.10 to 4.18% with an average of 2.4 .+-. 0.09 (S.D.) % which was significantly higher than those in normal controls (1.6 .+-. 0.29%). The values in the patients with Hashimoto's disease averaged 1.7 .+-. 0.53 (S.D.) which did differ significantly from those of controls. The value did not correlate with either TSH-binding inhibiting activities or titers of anti-microsomal antibodies. These data suggest the presence of TSH-receptor antibodies which react with antigens other than TSH-binding sites in the patients with Graves' disease.

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