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Binding of iodine 125 wheat germ agglutinin to chinese hamster ovary cells under conditions which affect the mobility of membrane components


, : Binding of iodine 125 wheat germ agglutinin to chinese hamster ovary cells under conditions which affect the mobility of membrane components. Journal of Cell Biology 79(3): 617-622

The binding of [125I]wheat germ agglutinin ([125I]WGA) of high specific activity to Chinese hamster ovary (CHO) cells was examined over a million-fold range of WGA concentrations and correlated with the phenomena of agglutination and capping by WGA. Analysis of the binding data by the method of Scatchard gave a complex curve indicative of positive cooperativity amongst high-affinity binding sites. Binding assays performed under conditions which inhibit capping and/or agglutination, such as low temperature or glutaraldehyde fixation, gave similarly complex binding curves. The gross mobility of WGA receptors in the membrane did not appear to be responsible for the cooperative binding of WGA to CHO cells.

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