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Biological and immunological studies of lipid containing neurophysin of rat and re aggregated lipid complex

, : Biological and immunological studies of lipid containing neurophysin of rat and re aggregated lipid complex. Biochimica et Biophysica Acta 572(3): 483-491

Biological and immunological properties of crude neurophysin, lipid-containing neurophysin and delipidated neurophysin protein mixture isolated from rat pituitary glands were investigated. Rat uterotonic, avian [rooster] vasodepressor and rat pressor assays indicate that crude neurophysin and lipid-containing neurophysin also contained 10-5 to 10-6 M neurohypophyseal hormones, with a 15-20 fold higher level of oxytocin as compared to vasopressin. Delipidated neurophysin had barely detectable hormonal levels. Electrolyte excretion studies also seem to indicate higher oxytocin levels in lipid-containing neurophysin. Lipid-containing neurophysin also contained biologically active ACTH (8 ng/.mu.g protein) and immunologically active ACTH-like material (20 ng/.mu.g protein). Excess immunoassayable ACTH-like material is converted to biologically active ACTH by trypsin treatment. Lipids isolated from rat pituitary were reaggregated in vitro with purified rat neurophysin-II to yield a neurophysin lipid complex. The amount of uptake of [2-14C(U[uniformly labelled]) Tyr]oxytocin by rat neurophysin-II or its neurophysin lipid complex was the same based on radioactivity. The specific avian vasodepressor activity of the oxytocin bound to the rat neurophysin-II lipid complex was enhanced approximately 3-fold.


PMID: 219896

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