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Busulfan and chloramphenicol induced T cell lymphoma: cell surface characteristics and functional properties


, : Busulfan and chloramphenicol induced T cell lymphoma: cell surface characteristics and functional properties. Clinical and Experimental Immunology 64(3): 646-655

We report the immunological studies on three transplantable lymphoma lines that developed when CAF1 mice were injected with busulfan and chloramphenicol. The lymphoma cells displayed Thy-1.2, brain associated antigen, and H-2d alloantigen. They were negative for surface IgM and Ia antigens. Expression of T cell differentiation antigens differed among the three lines. The 508 tumour line displayed only Thy-1.2; 408 tumour line displayed Thy-1.2, Lyt-2.2 and TL; and 808 tumour line was positive for Thy-1.2, Lyt-1.2, Lyt-2.2 and TL antigens. We established in vitro culture lines from 508 and 808 lymphoma cells. The lymphoma cells did not respond to mitogens and antigens. The splenic cells from mice bearing 508 or 808 had decreased phytohaemagglutinin (PHA), concanavalin A (Con A) and mixed leucocyte responses (MLR). When mitomycin-C treated lymphoma cells from the tumour bearing mice were cocultured with normal splenic mononuclear cells, the 808 lymphoma cells suppressed the mitogenic responses of the normal cells more profoundly than 508 lymphoma cells. Adherent cells from both tumours suppressed the Con A responses of normal spleen cells. Cells from in vitro 508 or 808 cell lines had no effect on mitogenic responses of normal cells. Plasma from tumour bearing mice, but not the supernatants taken from cultures of these lymphoma cells, suppressed the mitogenic responses of normal lymphocytes. Spleen cells from normal CAF1 mice responded in mixed leucocyte tumour reactions (MLTR) when cocultured with lymphoma cells. Mice immunized with mitomycin-C treated tumour cells had greater response. Responder cells taken from mice and established 508 or 808 tumors had suppressed MLTR responses. Although prior immunization with tumour antigen increased the MLTR response, injection of live tumour cells into immunized mice resulted in a more rapid tumour growth and suppression of MLTR response.

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PMID: 2947760


Other references

Dolan, N.C.; Feinglass, J.; Priyanath, A.; Sorensen, A.V.; Haviley, C.; Venta, L.A., 2000: Measuring the satisfaction with mammography results reporting A telephone survey of 298 patients. Journal of Investigative Medicine 48(5): 255A, September

Lahav, O.; Morgan, B.E., 2004: Titration methodologies for monitoring of anaerobic digestion in developing countries - a review. An increase in volatile fatty acids (VFA) concentration (or the proportional decrease in carbonate alkalinity concentration) is the first practical measurable indication that an anaerobic treatment system is in a state of stress. If the system is...

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Testet-Lamant, V.; Archaimbault, B.; Durand, J.; Rigaud, M., 1992: Enzymatic synthesis of structural analogs of PAF-acether by phospholipase D-catalysed transphosphatidylation. PAF-acether can be transformed into analogs by the phospholipase D enzyme activity of Streptomyces sp. In this reaction choline is replaced by primary cyclic alcohols (acceptors). The reaction products, cyclic phospholipid and phosphatidic acid, w...

Tremblay; Pepper; Newbury; Ritchie; Peacock; Frost; Jones; Hill, 1990: Low-field magnetotransport in p-type GaAs in the regime of variable-range-hopping conductivity. Physical Review. B, Condensed Matter 41(12): 8572-8575

Shipboard Scientific Party., 1991: Hole 810. Proceedings of the Ocean Drilling Program Initial Reports, 132: 75-111

Ziemianski, A., 1965: Galezie laczace (rami communicantes) nerwow rdzeniowych u psa. Folia Morphologica Warzawa, 1624: 323-339

Arikan, O.A.; Mulbry, W.; Rice, C., 2016: The effect of composting on the persistence of four ionophores in dairy manure and poultry litter. Manure composting is a well-described approach for stabilization of nutrients and reduction of pathogens and odors. Although composting studies have shown that thermophilic temperatures and aerobic conditions can increase removal rates of selected...

Mierzejewska, Z., 1975: Use of municipal sewage for agricultural irrigation. Nowe rolnictwo: 15, 24 (9) 17-18