geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Carbohydrate differentiation antigens of murine T cells: expression on intestinal lymphocytes and intestinal epithelium


, : Carbohydrate differentiation antigens of murine T cells: expression on intestinal lymphocytes and intestinal epithelium. Journal of Immunology 138(10): 3375-3384

Carbohydrate differentiation antigens (CT antigens) which previously had been shown to be associated with cytotoxic T cells were found at high levels on intestinal intraepithelial lymphocytes (IEL) and on the intestinal epithelium. Histological examination of intestinal sections demonstrated that the CT1 MAb defined epitopes on IEL and on epithelial cells located in the base of the villi crypts. The CT2 MAb reacted with IEL but also bound to the majority of cells in the intestinal epithelium. When isolated intestinal cell populations were analyzed by flow cytometry, two major size classes of cells were evident. The smaller cells, corresponding to lymphocytes, were primarily Lyt-2+, with a high proportion expressing CT antigens. Another differentiation antigen defined by the MAb J11d was absent from IEL, indicating that those IEL of T cell origin are likely to be mature because thymocytes, but not peripheral T cells, express the J11d antigen. Two-color fluorescence analysis indicated that the CT determinants were present on the Thy-1+, Lyt-2+, and the Thy-1-, Lyt-2+ subsets of IEL. However, the small percentage of L3T4+ IEL were CT-, further supporting our previous demonstration of a correlation between CT expression and Lyt-2 expression. Interesting phenotypic characteristics of IEL other than CT antigen expression were also detected. IEL did not express the MEL-14 lymphocyte homing receptor, and the cell surface level of LFA-1 was significantly lower than that of other peripheral lymphocytes. It was also shown that a small percentage of IEL express a T cell receptor allotypic marker, indicating that at least some of the cells are mature in terms of T cell receptor gene rearrangements. The large intestinal cells, although CT+, were not hematopoietic in origin because they were T200- and were shown by using chimeric mice not to be bone marrow-derived. In contrast to previously reported results, the cytotoxic activity of IEL was negligible with detectable lysis against NK-sensitive cells and other tumor cells, being observed in only one of seven experiments. Thus, the expression of the CT determinants was not indicative of cytotoxic ability, as previously suggested. The presence of specific carbohydrate residues on the cell surface of a subset of lymphocytes in an anatomically distinct immune compartment suggests that a unique differentiation pathway is followed by these cells.

US$29.90

PMID: 2437191


Other references

Zielińska, M.; Stafiej, A.; Law, R.O.; Albrecht, J., 2004: Effects of methionine sulfoximine on the glutamine and glutamate content and cell volume in rat cerebral cortical slices: involvement of mechanisms not related to inhibition of glutamine synthesis. Treatment of cerebral cortical slices with 5mM ammonium acetate (ammonia) elevated the glutamine (Gln) content and increased cell volume in the slices, in agreement with the postulated contribution of glutamine to hyperammonemic brain edema [Neuro...

Rhein, C., 2002: Entwicklung der Tierschutzrechtsetzung der EG. Animal welfare is of increasing importance for the European Community, which is reinforced by the Protocol to the EC Treaty on protection and welfare of animals. After the recent improvements to the legislation on the protection of pigs, the next...

Duckett S.; Galle P., 1976: Evidence of aluminum in senile plaques of alzheimer disease castaing micro probe study. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles 282(4): 393-395

Bacchus, M.Y.; Hay, D.M., 1970: Shirodkar suture. A review of 10 years' experience. American Journal of Obstetrics and Gynecology 108(2): 250-252

Sikdar, S.; Shah, J.P.; Gilliams, E.; Gebreab, T.; Gerber, L.H., 2009: Assessment of myofascial trigger points (MTrPs): a new application of ultrasound imaging and vibration sonoelastography. Myofascial trigger points (MTrPs) are palpable hyperirritable nodules in skeletal muscle that are associated with chronic musculoskeletal pain. The goal of this study was to image MTrPs in the upper trapezius muscle using 2D gray scale ultrasound...

Caignard, A.; Dietrich, P.Y.; Morand, V.; Lim, A.; Pannetier, C.; Leridant, A.M.; Hercend, T.; Even, J.; Kourilsky, P.; Triebel, F., 1994: Evidence for T-cell clonal expansion in a patient with squamous cell carcinoma of the head and neck. Squamous cell carcinomas of the head and neck (SCCHN) often contain a large mononuclear cell infiltrate, composed mainly of T-lymphocytes which could reflect an in situ immune reaction against the malignant SCCHN cells. We analyzed the molecular s...

Sharshunov, V.; Kulagin, V., 1980: Friction coefficient of briquetted feeds Strength during transportation.1. Mekhanizatsiia i elektrifikatsiia sel'skogo khoziaistva: (7) 55-56

Hogan, A.G.; Richardson, L.R., 1936: Differentiation Of The Antidermatitis Factor. IV. Experiments on rats showed that dermatitis produced by feeding a basal ration deficient in all members of the vitamin B complex, supplemented by a vitamin B carrier exposed to ultra-violet irradiation, was healed by the addition of wheat-germ...

Van Kalmthout, J.M., 1954: Investigations on the physiology of dicumarol. When human oxalated normal plasma is refrigerated, the prothrombin time increased. On the contrary, the prothrombin time of oxalated Dicumarol-plasma decreased when stored under similar conditions, provided the time was sufficiently prolonged. Thi...

Costa, M.A., 1943: Parasitosis intestinal en el nino. RevChile: 41- 48