geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Carbon 13 nmr study of 85 percent carbon 13 enriched proline thyrotropin releasing factor carbon 13 carbon 13 vicinal coupling constants and conformation of the proline residue


, : Carbon 13 nmr study of 85 percent carbon 13 enriched proline thyrotropin releasing factor carbon 13 carbon 13 vicinal coupling constants and conformation of the proline residue. Proceedings of the National Academy of Sciences of the United States of America 72(12): 4948-4952

To understand fully interactions between peptides and cellular receptors, peptide side chain conformation must be defined. In many cases the complexity of proton NMR prevents this, but the present work demonstrates this problem can be solved by using 13C enrichment. Selective 13C enrichment of a natural peptide hormone was achieved by preparing [85% 13C-enriched proline]thyrotropin releasing factor which was examined by 13C NMR spectroscopy at various pH values. Because of the 13C enrichment, 1-bonded and 3-bonded (vicinal) 13C-13C coupling constants were determined. The latter vary from 0-5 Hz and show bond angle dependence. In this hormone the pyrrolidine ring is not free but fixed in the C.gamma.-endo puckered conformation. It has also been possible to assign chemical shift values for a second order 13C NMR spectrum.

US$29.90


Other references

Casagranda, F.; Wilshire, J.F., 1997: Enzymatic and chemical methods for manual C-terminal peptide sequencing. Methods in Molecular Biology 64: 243-257

Park, T.; Yusuf, A.A.; Hadsall, R.S., 2016: Pharmacy Students' Attitudes Toward Debt. To examine pharmacy students' attitudes toward debt. Two hundred thirteen pharmacy students at the University of Minnesota were surveyed using items designed to assess attitudes toward debt. Factor analysis was performed to identify common th...

Matteis, M.; Vernieri, F.; Caltagirone, C.; Troisi, E.; Rossini, P.M.; Silvestrini, M., 1999: Patterns of cerebrovascular reactivity in patients with carotid artery occlusion and severe contralateral stenosis. There is evidence suggesting that among other factors, an alteration in cerebral hemodynamics plays a relevant role in the occurrence of strokes in patients with carotid disease. The purpose of this study was to investigate patterns of cerebrovasc...

Navarro E.; Graffigna A.; Trujillo J.; Boada J., 1989: Cardioactive glucosides of cheiranthus scoparius brous. Revista de Farmacologia Clinica y Experimental 6(3): 239

Semple, T.U.; Moore, G.E.; Morgan, R.T.; Woods, L.K.; Quinn, L.A., 1982: Multiple cell lines from patients with malignant melanoma: morphology, karyology, and biochemical analysis. Of 23 cell lines representing 8 patients with malignant melanoma, one cell line from each patient has been extensively characterized by means of phase morphology, ultrastructural morphology, biochemical markers, steroid receptor protein analysis,...

Hess N., 1969: Vascular ischemic peripheral neuropathy. Schweizer Archiv fuer Neurologie Neurochirurgie und Psychiatrie 105(1): 1-17

Pospelov I.N., 2001: Natural features of Levinson-Lessing Lake basin, central Taymyr. Cryomorphogenic processes and cryogenic relief of Levenson-Lessing lake basin (Byrranga Mountains, central Taimir) are described. The regional dynamic scheme of morphogenetic process development of the territory as three rows--watershed-slope, val...

Liu, C.; Chen, X., 2014: A potential cellular host factor homologous to ZNF-136 can interact with truncated nonstructural protein 2 of hepatitis C virus. The cytoplasmic portion of the NS2 protein of hepatitis C virus (HCV) fused with a thioredoxin (TrX)-His tag was expressed in BL21 (DE3) cells and purified using an Ni-NTA His resin. Using a pull-down assay, a specific protein from the cell lysate...

Collins, Christine A., 1994: Dynein-based organelle movement. Hyams, J S [Editor], Lloyd, C W [Editor] Modern Cell Biology; Microtubules 367-380

Pehap A., 1973: Study of some fractions preliminarily called purine derivatives in pinus sylvestris seed endosperm. Institutionen for Skogsforyngring Rapporter och Uppsatser 46: 67-121