+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cartilage proteoglycan core protein gene expression during limb cartilage differentiation

, : Cartilage proteoglycan core protein gene expression during limb cartilage differentiation. Developmental Biology 118(1): 112-117

Changes in the steady-state cytoplasmic levels of mRNA for the core protein of the major sulfated proteoglycan of cartilage were examine during the course of limb chondrogenesis in vitro using cloned cDNA probes. Cytoplasmic core protein mRNA begins to accumulate at the onset of overt chondrogenesis in micromass culture coincident with the crucial condensation phase of the process, in which prechondrogenic mesenchymal cells become closely juxtaposed prior to depositing a cartilage matrix. The initiation of core protein mRNA accumulation coincides with a dramatic increase in the accumulation of mRNA for type II collagen, the other major constituent of hyaline cartilage matrix. Following condensation, there is a concomitant progressive increase in cytoplasmic core protein and type II collagen mRNA accumulation which parallels the progressive accumulation of cartilage matrix by the cells. The relative rate of accumulation of cytoplasmic type II collagen mRNA is greater than twice that of core protein mRNA during chondrogenesis in micromass culture. Cyclic AMP, an agent implicated in the regulation of chondrogenesis elicits a concomitant two- to fourfold increase in both cartilage core protein and type II collagen mRNA levels by limb mesenchymal cells. Core protein gene expression is more sensitive to cAMP than type II collagen gene expression. These results suggest that the cartilage proteoglycan core protein and type II collagen genes are coordinately regulated during the course of limb cartilage differentiation, although there are quantitative differences in the extent of expression of the two genes.


PMID: 3021549

DOI: 10.1016/0012-1606(86)90078-3

Other references

Barbato, A.; D'Avanzo, B.; Rocca, G.; Amatulli, A.; Lampugnani, D., 2003: A study of long-stay patients resettled in the community after closure of a psychiatric hospital in Italy. This study examined the outcomes of all patients who were discharged from an Italian psychiatric hospital into community residences three to four years after discharge. The total population of Antonini Mental Hospital near Milan on January 1996 (N...

Reisch, J.; el-Sharaky, A.S., 1985: The chemistry of natural products. 93. Detection of 1-O-alkylglycerins in lipid extracts of the adult human thymus. Archiv der Pharmazie 318(11): 989-991

Scobie, H.M.; Rainey, G.Jonah.A.; Bradley, K.A.; Young, J.A.T., 2003: Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Bacillus anthracis secretes two bipartite toxins thought to be involved in anthrax pathogenesis and resulting death of the host. The current model for intoxication is that protective antigen (PA) toxin subunits bind a single group of cell-surface...

Weinberg, Z.G.; Ashbell, G.; Azrieli, A., 1988: The effect of applying lactic bacteria at ensilage on the chemical and microbiological composition of vetch, wheat and alfalfa silages. The effect of applying a commercial lactic acid bacterial inoculant at 5.6 x 104 cfu/g fresh material to Vicia sp., wheat, direct cut and wilted Medicago sativa silages was studied under laboratory conditions, and on wheat also under farm conditio...

Stibler, H.; Sydow, O.; Borg, S., 1980: Quantitation of a qualitative change of transferrin heterogeneity in alcoholics. Drug and Alcohol Dependence 6(1): 11-12

Beilin, Y.; Leibowitz, A.B.; Haddad, E.M.; Bodian, C.A., 1995: Clinical use of the bleeding time test in obstetric anesthesia. Anesthesiology (Hagerstown) 83(3A): A1057

Thraehnart O.; Kuwert E.K.; Scheiermann N.; Dermietzel R.; Paar D.; Maruhn D.; Alberti A.; Richter H J.; Hotz J., 1982: Comparison of the morphological alteration and disintegration test and the chimpanzee infectivity test for determination of hepatitis b virucidal activity of chemical disinfectants. The morphological alteration and disintegration test (MADT) as a key indicator for hepatitis B virus (HBV) inactivation was compared with the chimpanzee infectivity test using 3 suspensions of HBV differing in the degree of inactivation as judged...

Brustis J J.; Galante M.; Peyret D., 1982: Evolution of adenylate cyclase activity in the cordomesoderm and the endoderm during primordial germ cells migration in xenopus laevis embryo. In Xenopus laevis embryo adenylate cyclase activity is higher in the cordomesoderm than in the endoderm. The peak of activity in the cordomesoderm is observed at the beginning of the migration of the primordial germ cells (PGC). There could be a c...

Ward, A.A., 1947: Convulsive activity induced by fluoroacetate. Intravenous injn. of Na fluoroacetate causes local, rhythmic seizure discharges in subcortical structures. These occur at the time when the animal is exhibiting repeated, tonic seizures. There is no clear correlation between the electrical activit...

Belous L.I.; Sboeva S.G., 1986: Comparative analysis of the use of the local resources of medicinal plant raw material by pharmacies. Farmatsiya (Moscow) 35(4): 11-14