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Cellular localization and properties of glutaminase in aspergillus oryzae


, : Cellular localization and properties of glutaminase in aspergillus oryzae. Nippon Nogeikagaku Kaishi 59(3): 245-252

Of the intracellular activity of glutaminase in A. oryzae No. 27, .apprx. 30% was found in free form and the remaining activity in bound form. During protoplast formation, mycelium glutaminase was released in the solubilized cell wall fraction. Glutaminase activity in lysed protoplasts was .apprx. 500-fold lower than that found in the solubilized cell wall fraction. These data indicate that most glutaminase was located at the cell surface, including the cell wall and the periplasm. Therefore, intracellular free glutaminase exists in the periplasm or is bound lossely to the cell wall and the intracellular bound glutaminase is bound tightly to the cell wall. The intracellular free form of glutaminase was definitively different from intracellular bound glutaminase in sensitivity to NaCl and NaF, and in recovery after acetone treatment at different pH values.

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