+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of cytosine permeation in Saccharomyces cerevisiae

, : Characterization of cytosine permeation in Saccharomyces cerevisiae. Journal of Bacteriology 122(2): 629-641

Cytosine permeation in Saccharomyces cerevisiae has been studied. Cytosine uptake is mediated by a permease which is also responsible for purines transport. The Km for the transport of various substrates of this permease have been determined. By means of appropriate selective techniques, mutants with altered Km and mutants lacking the permease have been selected. Cytosine transport is active and is inhibited by 2,4-dinitrophenol, an uncoupler of oxidative phosphorylation, and by N-ethylmaleimide, a reagent of--SH group. Internal labeled cytosine is chased by addition of unlabeled cytosine in the medium. These results support the hypothesis of a carrier-mediated transport, with reduced internal affinity, allowing the release and accumulation of cytosine in the inner compartment. The efflux of cytosine from cytosine permease-less cells has also been studied and shows first order kinetics. A diffusion coefficient of 5.7 per 10- minus 8 cm per S- minus 1 has been evaluated for this efflux.


PMID: 47858

Other references

Odell, W.D.; Griffin, J.; Sawitzke, A., 1990: Chorionic gonadotropin secretion in normal, nonpregnant humans. A chorionic gonadotropinlike material is present in blood and urine of normal, nonpregnant humans, is secreted in pulsatile fashion in parallel with luteinizing hormone, is stimulated by gonadotropin-releasing hormone, and is suppressed by GnRH ag...

Mel' nikov, M.T.; Balan, V.I.; Shatalov, S.V.; Semenyak, V.I., 1988: Feed cellolignin in diets for sheep. Cellolignin added to the diet of sheep to replace part of hay and barley meal saved 7 to 9% of the replaced feeds.

Katz, A.R.; Lee, M.Veneranda.C.; Ohye, R.G.; Effler, P.V.; Johnson, E.C.; Nishi, S.M., 2004: Prevalence of chlamydial and gonorrheal infections among females in a juvenile detention facility, Honolulu, Hawaii. Limited studies have shown high rates of gonorrheal and chlamydial infections among incarcerated adolescents, however, this population has not been routinely targeted for sexually transmitted disease (STD) screening. This study was done to ascerta...

Dubar Michel, 1980: Pleistocene terrains of the Nice region. G27: 44-45 1980

Yu, P.; Huang, L.; Li, H.; Liu, M.; Zong, J.; Li, C.; Chen, F., 2016: Epidemiological investigation of an outbreak of hepatitis A in rural China. This investigation was a response to an outbreak of hepatitis A in rural China in 2013. The objectives were to identify the pattern of transmission and the risk factors. A probable case was defined as an individual in/nearby the village of the out...

Roupret, M.; Rozet, Fçois.; Sanderson, K.M.; Lesur, G.; Almeida, D.; Harmon, J.D.; Barret, E.; Cathelineau, X.; Vallancien, G., 2006: Place of laparoscopic nephroureterectomy in the treatment of upper urinary tract transitional cell carcinoma. The current reference treatment for upper urinary tract transitional cell carcinoma is open nephroureterectomy via a lumbar incision and an iliac incision with resection of a large bladder cuff: Since the first laparoscopic nephroureterectomy perf...

Freyne, A.; Byrne, B., 2013: To screen or not to screen for subclinical hypothyroidism in pregnancy?. Irish Medical Journal 106(6): 165-165

van der Linden, H.; Kalra, D.; Hasman, A.; Talmon, J., 2008: Inter-organizational future proof EHR systems. A review of the security and privacy related issues. Identification and analysis of privacy and security related issues that occur when health information is exchanged between health care organizations. Based on a generic scenario questions were formulated to reveal the occurring issues. Possible an...

Marin, G., 2001: Silvopastoral system in Romania. The silvopastoral system in Romania originates back since 1921-1923 when, according to the Reform Agricultural Law, about 1 million hectares of forests from the mountain areas have been transferred from the forest fund in order to the pastoral gra...

Mishra P.S.; Dutta K.K., 1984: Micro-facies of evaporite cyclothems from Simrawal Shale Formation of central Vindhyan Basin, Rewa Plateau, Madhya Pradesh. Records of the Geological Survey of India: Part 6; Pages 82-98. 1984.