geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of cytoskeletal components in epithelial and mesenchymal liver tumors by electron microscopy and immuno fluorescence microscopy


, : Characterization of cytoskeletal components in epithelial and mesenchymal liver tumors by electron microscopy and immuno fluorescence microscopy. Virchows Archiv B Cell Pathology Including Molecular Pathology 36(2-3): 139-158

Intermediate-sized filaments and microfilaments were studied by immunofluorescence and EM in rat liver tumors induced by nitrosamines. Hepatocellular carcinomas contained tonofilaments and microfilaments which could be demonstrated light microscopically by antibodies to prekeratin and actin, respectively. These were predominantly localized in peripheral cytoplasmic regions. The hepatocellular tumors did not show significant amounts of intermediate-sized filaments of the vimentin-type. The epithelial duct component of cholangiofibromas exhibited actin-positive microfilaments organized in a terminal web and prekeratin-positive tonofilaments, the latter often being present in large amounts. The mesenchymal component of the cholangiofibromas and the cells of angiosarcomas or undifferentiated sarcomas were characterized by intermediate vimentin filaments but not perkeratin. Mesenchymal tumors contained small amounts of actin. Many sarcoma cells contained a pronounced accumulation of intermediate-sized filaments of the vimentin type, frequently in whorl-like aggregates. The characteristic vimentin storage cells were pathognomonic for sarcomas, especially for angiosarcomas. The detection of early stages of angiosarcomas by antibodies to vimentin was facilitated. The presence of an excess of contractile proteins is apparently not an important prerequisite for the metastasic spread of malignant cells. Antibodies to the different classes of intermediate filaments are a powerful tool for the classification and differential diagnosis of epithelial and mesenchymal tumors of the liver. The accumulation of prekeratin in many ductular cells of the cholangiofibromas and the considerable storage of vimentin in sarcoma cells seem to indicate a severe disturbance of the cell metabolism which might be related to the neoplastic transformation.

US$29.90


Other references

Baillie, J., 1991: Needle knife sphincterotomy. Gastrointestinal Endoscopy 37(6): 650-650

De-Jesus-Dematei-Gregio, S.; Moscheta, I.-Sebastiao, 2006: Anatomia de raiz, caule e folha e identificacao de estruturas secretoras de Achillea millefolium L. (Asteraceae). Art anatomic study of the vegetative organs: root, stem and leaf was made. The essential oils were extracted from the plant identifying the possible secretory structures. Fresh material was used in order to perform histochemical tests and to prep...

Hussey, D.K.; Madanat, R.; Donahue, G.S.; Rolfson, O.; Bragdon, C.R.; Muratoglu, O.K.; Malchau, H., 2016: Scoring the Current Risk Stratification Guidelines in Follow-up Evaluation of Patients After Metal-on-Metal Hip Arthroplasty: A Proposal for a Metal-on-Metal Risk Score Supporting Clinical Decision-Making. In the follow-up evaluation of patients with metal-on-metal (MoM) hip replacements, current evidence suggests that orthopaedic surgeons should avoid reliance on any single investigative tool. Current risk stratification guidelines can be difficult...

Schmaderer, C.; Xing, C.Jiang.; Anderson, G.; Hermans, R.; Lutz, J.; Heemann, U.; Baumann, M., 2011: AGE formation blockade with aminoguanidine does not ameliorate chronic allograft nephropathy. Glycation end products (AGEs) are produced by glycoxidation and lipid peroxidation. AGEs induce oxidative stress and inflammation, and accumulate in tubular cells after kidney transplantation. We hypothesize that the AGE formation blocker aminogua...

Dent, K.M.; Dunn, D.M.; von Niederhausern, A.C.; Aoyagi, A.T.; Kerr, L.; Bromberg, M.B.; Hart, K.J.; Tuohy, T.; White, S.; den Dunnen, J.T.; Weiss, R.B.; Flanigan, K.M., 2005: Improved molecular diagnosis of dystrophinopathies in an unselected clinical cohort. Mutations in the DMD gene result in Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD). Readily available clinical tests detect only deletions of one exon or greater, which are found in approximately 60% of cases. Mutational ana...

Goren, E.; Engelberg, I.S.; Eidelman, A., 1991: Adrenal rest carcinoma in hilum of kidney. Adrenocortical rests are uncommon in adults and infrequently undergo malignant changes. Most of the published cases are those of functional tumors allowing preoperative diagnosis on the basis of the resulting endocrinopathy. Most nonfunctioning tu...

Wong, S.; Sanchez, T.Ray.S.; Swischuk, L.E.; Huang, F.S., 2008: Diffuse peritoneal lymphomatosis: atypical presentation of Burkitt lymphoma. Burkitt lymphoma presenting as a peritoneal and small thickening problem mimicking carcinomatosis is very rare. We present a previously healthy 7-year-old boy with a 1-week history of worsening abdominal distention, vomiting and melena. He demonst...

Cerin, E.; Sit, C.H.P.; Barnett, A.; Huang, W.Y.J.; Gao, G.Y.; Wong, S.H.S.; Sallis, J.F., 2017: Reliability of self-report measures of correlates of obesity-related behaviours in Hong Kong adolescents for the iHealt(H) and IPEN adolescent studies. This study examined the reliability of measures of correlates of dietary behaviours (DBs), physical activity (PA) and sedentary behaviour (SB) for Hong Kong adolescents. Individual, social and environmental correlates of obesity-related behaviours...

Baronnet Alain, 1997: Equilibrium and kinetic processes for polytype and polysome generation. European Mineralogical Union Notes in Mineralogy: ges 119-152. 1997.

Batuwita, S.; Alagiyawadu, I., 2004: Some observations of Hemiphyllodactylus typus Bleeker, 1860: Sri Lanka (Squamata: Gekkonidae). Lyriocephalus. February; 51-2: 146-149 (Special issue)