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Characterization of poly adp ribose polymerase activity in nuclei from the slime mold dictyostelium discoideum

, : Characterization of poly adp ribose polymerase activity in nuclei from the slime mold dictyostelium discoideum. Molecular & Cellular Biochemistry 27(2): 79-84

Nuclei isolated from D. discoideum and incubated in vitro with 3H-NAD synthesize poly(ADP-Rib). The optimum incubation conditions for the poly(ADP-Rib) polymerase were determined. The Km of the enzyme is 18 .mu.M NAD and it is inhibited by nicotinamide. Most of the poly(ADP-Rib) synthesized is attached to nuclear proteins.


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