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Characterization of the dna species of 5 baculoviruses pathogenic for the armyworm pseudaletia unipuncta


, : Characterization of the dna species of 5 baculoviruses pathogenic for the armyworm pseudaletia unipuncta. Journal of Invertebrate Pathology 46(2): 174-179

Five baculoviruses, 3 granulosis (GV), and 2 nuclear polyhedrosis (NPV) viruses which infect P. unipuncta were analyzed with 4 restriction enzymes. All viruses gave unique restriction fragment patterns even though each progency virus had been produced in larvae of the same insect species. Hybridization of the restriction fragments, immobilized on nitrocellulose, with 32P-labeled viral DNA probes showed no homology between the Oregonian GV and the other 2 GVs, nor between the 2 NPVs. Some homology, however, was detected between the Hawaiian GV and the GVS isolated from Scotogramma trifolia, both of which produce similar but distinct synergistic factors that enhance baculovirus infections. The results present unequivocal identity of the 5 baculoviruses and also provide a basis for detecting recombinants of these viruses.

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