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Comparative determination of rna polymerase activity and rna polymerase b concentration in chromatin preparations from myxomycetes physarum polycephalum

, : Comparative determination of rna polymerase activity and rna polymerase b concentration in chromatin preparations from myxomycetes physarum polycephalum. Biokhimiya 44(12): 2170-2174

A titration procedure for endogenous RNA-polymerase B in chromatin preparations from the plasmodium of P. polycephalum grown for 50 and 70 h was developed. The enzyme concentration in the preparations determined by this method was equal to (1.38 .+-. 0.038) .cntdot. 10-11 M and (2.9 .+-. 0.19). .cntdot. 1011 M, respectively. These values do not correlate with the values of RNA-polymerase activity determined under standard conditions. Possible mechanisms of inhibition of enzyme activity at various stages of myxomycetus growth are discussed. The inhibition may be possibly connected with the mechanism of transcription.


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