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Comparative studies of solid phase method and double antibody method in carcino embryonic antigen radio immunoassay


, : Comparative studies of solid phase method and double antibody method in carcino embryonic antigen radio immunoassay. Bulletin of the Faculty of Agriculture Yamaguchi University (31): 55-72

The solid phase method and double antibody method in [human] CEA [carcinoembryonic antigen] radioimmunoassay were compared. The amount of serum needed for the assay was larger in the solid phase method than in the double antibody method. Before the assay procedure, preparation of various reagents was required for the double antibody method, but not for the solid phase method. Extraction of CEA was required for the solid phase method, but not for the double antibody method. Incubation temperature must be kept at 20.degree. C in the double antibody method, but room temperature is suitable for the solid phase method. The assay was completed in 1-2 days in the solid phase method, but 2-3 days was required for the double antibody method. To express the binding ability in standard curve, B/T was adequate in the solid phase method, but B/Bo was suitable in the double antibody method. The standard curve was almost correctly fitted from 1-100 ng/ml in the solid phase method and was nearly correctly fitted from 5-160 ng/ml in the double antibody method. With regards to accuracy, reproducibility and recovery, good results were obtained with both methods. The solid phase and double antibody methods were equally useful for the CEA assay.

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