+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Comparison of analytical methods for the estimation of glycosylated hemo globins

, : Comparison of analytical methods for the estimation of glycosylated hemo globins. Journal of Clinical Chemistry & Clinical Biochemistry 19(11): 1097-1102

The glycosylated Hb of 26 different blood samples from diabetic patients were analyzed by 6 different methods; the regression lines and the correlation coefficients were calculated from the analytical data. The best separation and estimation of the fast Hb fractions were achieved by high performance liquid chromatography (HPLC). Therefore HPLC is regarded as the reference method for the analysis of fast Hb fractions. Microcolumn chromatography or the thiobarbituric acid method are recommended for routine analysis in clinical laboratories. Since the labile aldimine (Schiff's base) can be calculated from the analytical data or destroyed by appropriate methods, this glycosylated Hb fraction does not interfere with the results.


PMID: 7310330

Other references

Ando S.; Isobe M.; Nagai Y., 1976: High performance preparative column chromatography of lipids using a new porous silica iatrobeads part 1 separation of molecular species of sphingo glyco lipids. Biochimica et Biophysica Acta 424(1): 98-105

Laurence, J.; Haller, H.; Mannucci, P.Mannuccio.; Nangaku, M.; Praga, M.; Rodriguez de Cordoba, S., 2016: Atypical hemolytic uremic syndrome (aHUS): essential aspects of an accurate diagnosis. NlmCategory="UNASSIGNED">Atypical hemolytic uremic syndrome (aHUS), a thrombotic microangiopathy (TMA), is a rare, life-threatening, systemic disease. When unrecognized or inappropriately treated, aHUS has a high degree of morbidity a...

Wahl, P.; Tawada, K.; Auchet, J.C., 1978: Study of tropo myosin labeled with a fluorescent probe by pulse fluorometry in polarized light interaction of that protein with troponin and actin. Tropomyosin from rabbit skeletal muscle was labeled with a fluorescent probe, N-iodoacetyl-N'-(5-sulfo-1-naphthyl)-ethylenediamine, which is presumed to bind preferentially to the unique reactive cysteine residue of the .alpha. chain. Anisotr...

Nilsen, D.W.; Gøransson, L.; Larsen, A.I.; Hetland, O.; Kierulf, P., 1997: Systemic thrombin generation and activity resistant to low molecular weight heparin administered prior to streptokinase in patients with acute myocardial infarction. One hundred patients were included in a randomized open trial to assess the systemic factor Xa (FXa) and thrombin inhibitory effect as well as the safety profile of low molecular weight heparin (LMWH) given subcutaneously in conjunction with strep...

Hayasaka, S., 1986: Hereditary congenital nystagmus. A Japanese pedigree. A Japanese pedigree of congenital nystagmus compatible with an X-linked irregular dominant transmission is described. An absence of male-to-male transmission, and skipping of a generation were noted. Genetic counseling for congenital nystagmus is...

Gross, A.S.; Hoskins, J.M.; Shenfield, G.M., 1997: Relationship between CYP2C19 genotype and proguanil metabolic ratio in Chinese. European Journal of Clinical Pharmacology 52(SUPPL ): A74

Atsumi, K., 1971: Present status and the future of artificial organs. Nihon Ishikai Zasshi. Journal of the Japan Medical Association 65(4): 423-435

Kapsokalyvas, D.; van Hoof, M.; Wigren, S.; Chimhanda, T.; Kuijpers, H.J.; Ramaekers, F.C.S.; Stokroos, R.J.; van Zandvoort, M.A.M.J., 2017: Investigating the race for the surface and skin integration in clinically retrieved abutments with two-photon microscopy. Bone conduction hearing implants can rehabilitate some types of hearing loss. A hydroxyapatite (HA)-coated skin-penetrating abutment was developed to allow for soft tissue preservation and increased skin-abutment adherence. Inflammation is thought...

Hummel, J.W., 1983: Impact cutting of soybeans using flexible cutting systems. Objectives of the study were: to measure the plant stem accelerations of soyabean plants and the harvest losses during impact cutting with flexible rotary blades and belt-mounted blades. Laboratory equipment and methods used to simulate a floating...

Borger, G.; Burke, S., 1994: Health reform's other woman. U.S. News & World Report 116(12): 38-38