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Comparison of the iso electric and molecular weight properties of tubulin subunits


, : Comparison of the iso electric and molecular weight properties of tubulin subunits. Journal of Neurochemistry 28(4): 697-706

Brain tubulin subunits were separated by a combination of isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate (SDS) using a 2-dimensional polyacrylamide slab gel technique. Isoelectric focusing separated tubulin subunits into 2 major groups of bands, such that the more acidic group corresponded to the .alpha. subunit and the less acidic group corresponded to the .beta. subunit. In addition, isoelectric focusing resolved the .beta. subunit into 2 subspecies which differed slightly in isoelectric properties but were the same apparent MW. The .alpha. subunit was resolved into many subspecies that appear to differ from each other by apparent MW and isoelectric properties.

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PMID: 894278


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