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Competence in escherichia coli cells part 5 interaction of the competence factor with cells/

, : Competence in escherichia coli cells part 5 interaction of the competence factor with cells/. Genetika 14(1): 163-170

Some trends of the interaction of the competence factor with the cells are described on the model od phage .lambda. transfection. This process depends on the composition of the recipients growth media, the temperature regime in the interaction process, and on the concentration of the competence factor and the recipient cells. The preliminary treatment of the recipient by EDTA (0.0001 M), CaCl2 (< 0.02 M), MgCl2 (< 0.01 M) and NaCl (< 0.01 M) resulted in the increase of their sensitivity to subsequent treatment by the factor. After the treatment of cells by the competence factor, their sensitivity to decreased CaCl2 concentration is increased.


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