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Conditional lethal mutants of adenovirus type 2 sv 40 hybrids part 2 ad 2 plus nd 1 host range mutants that synthesize fragments of the ad 2 plus nd 1 30k protein

, : Conditional lethal mutants of adenovirus type 2 sv 40 hybrids part 2 ad 2 plus nd 1 host range mutants that synthesize fragments of the ad 2 plus nd 1 30k protein. Journal of Virology 19(2): 559-571

Adenovirus type 2 (Ad2) grows 1000 times less well in monkey cells than in human cells. This defect can be overcome upon co-infection of cells with SV40 and also when the relevant part of the SV40 genome is integrated into the adenovirus genome to form an adenovirus-SV40 hybrid virus. The nondefective Ad2-SV40 hybrid virus Ad2+ND1, which contains an insertion of 17% of the SV40 genome, was used to isolate host-range mutants which are defective in growth on [African green] monkey [kidney CV-1] cells although they grow normally on human [HeLa] cells. Like Ad2, these mutants are defective in the synthesis of late proteins in monkey cells. A 30,000-MW protein (30K), unique to Ad2+ND1-infected cells, can be synthesized in vitro, using Ad2+ND1 mRNA that contains SV40 sequences. 30K is not seen in cells infected with those host-range mutants that are most defective in growth on monkey cells, and translation in vitro of SV40-specific mRNA from these cells produces new unique polypeptides instead of 30K. Genetic and biochemical analyses indicate that these mutants carry point mutations rather than deletions.


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Kolesnikov I.S.; Vikhriev B.S.; Lesnitskii L.S., 1972: Thoraco centesis and drainage in treatment of patients with pulmonary abscesses. Vestnik Khirurgii Imenii I I Grekova 108(1): 13-16

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Antlsperger, D.S.M.; Dirsch, V.M.; Hentze, H.; Vollmar, A.M., 2001: Mechanism of ajoene-induced apoptosis in promyelo-leukemic cells. Naunyn-Schmiedeberg's Archives of Pharmacology 363(4 Supplement): R84

Paiva, M.P., 1969: Estudo sobre a pesca de lagostas no Ceara, durante o ano de 1968. Archos Cienc Mar, 9: 41-55

Shabman, L., 1997: Making benefit estimation useful: lessons from flood control experience. Water resources update mn (109) 19-24

McNamara, D.G., 1975: What the pediatrician should know about congenital heart disease. Paediatrica Indonesiana 15(1-2): 55-68

Denahan, T.; Barney, J.A.; Sheldahl, L.M.; Ebert, T.J., 1993: Lack of changes in cardiac-vagal activity in older males following 12 weeks of aerobic training. Medicine & Science in Sports & Exercise 25(5 SUPPL ): S55

Stoltenberg, E., 1977: Analysis of the village as a place to live. The GFR Regional Planning Report of 1974 saw its main task as the creation of equal living conditions in all parts of the GFR so as to prevent a further depopulation of rural areas. The paper discusses the definition of a rural area and describes...

Czarnomska, A.; Démant, P., 1975: A new H-2 haplotype carrying possibly a mutation of H-2b. Folia Biologica 21(6): 419-420

Levine, N.D.; Morrill, C.C.; Schmittle, S.C., 1950: Renal coccidiosis in an Illinois gosling. North American Veterinarian 31(11): 738-739