+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Conformational effects of organic solvents on histone complexes

, : Conformational effects of organic solvents on histone complexes. Biochemistry 21(8): 1765-1772

Changes in the conformations of H3-H4, H2A-H2B and the core histone complex [from chicken erythrocyte nuclei] brought about by the addition of organic solvents were examined by circular dichroism spectroscopy. All 3 complexes assume increased .alpha. helicity with increasing amounts of the organic solvent. An amount of secondary structure equal to that obtained in phosphate-buffered 2 M NaCl solution can be induced in low-salt solutions of the complexes by the addition of 40-50% ethylene glycol, 50% glycerol, or .apprx. 2% hexafluoro-2-propanol. H3-H4 was somewhat more flexible than H2A-H2B in its response to changes in solvent polarity. Upon being heated, H3-H4 and the core histone complex both undergo irreversible .alpha. .fwdarw. .beta. transitions in 50% ethylene glycol under low-salt conditions, while H2A-H2B undergoes an essentially reversible .alpha. .fwdarw. random-coil transition under the same conditions. These results are discussed in terms of the dynamics of the nucleosome particle.


PMID: 7082646

DOI: 10.1021/bi00537a011

Other references

Pokorny, R.; Porubova, M., 2001: Resistenz von maislinien und -hybriden gegen Tschechische isolate von Maize dwarf mosaic virus und Sugarcane mosaic virus. Under greenhouse conditions, 65 maize lines were evaluated for resistance to isolates MDMV 91Tr and SCMV 190Tr. Thirty eight days after inoculation by MDMV, most plants of 42 lines developed symptoms of infection (80-100%), but individual lines di...

Arakaki, P.Rocha.; Carvalho,; Castro,; Muniz, Jé.Augusto.Pereira.Carneiro.; Valle,, 2017: Collection, Evaluation, and Coagulum Dissolution of Semen from Goeldi's Monkey, Callimico goeldii. Research on Neotropical primates' reproduction is necessary due to the lack of available information and the increasing threat to these species. Callimico goeldii is listed as Vulnerable on the IUCN Red List of Threatened Species. This study...

Anonymous, 1978: Plan targets of agriculture and food industry for l977. Allattenyesztes 7 (1) 1-7

Pokorny, V., 1968: Baltraella batraensis gen n, sp n Bythocytheridae, Ostracoda from Recent deposits of the Galapagos islands. Casopis pro Mineralogii a Geologii, 13: 385-390

Berridge, M.J., 1985: Phosphoinositides and signal transductions. Reviews in Clinical & Basic Pharmacology 5 Suppl: 5s-13s

Aresta, M.; Dibenedetto, A.; Angelini, A., 2013: The use of solar energy can enhance the conversion of carbon dioxide into energy-rich products: stepping towards artificial photosynthesis. The need to cut CO₂ emission into the atmosphere is pushing scientists and technologists to discover and implement new strategies that may be effective for controlling the CO₂ atmospheric level (and its possible effects on climate change). One...

Lebedev, N.N.; Shikhmetov, A.N.; Dubovik, V.A., 2016: The technologies of inpatient care substitution in surgery. Цель - анализ работы по внедрению стационарзамещающих технологий в многопрофильной Поликлинике ОАО «Газпром». Материал и методы. Изло...

L.F.r, C.; Legeret, B.; de Sainte Claire, P.; Wong-Wah-Chung, P.; Sarakha, M., 2013: Liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry for the analysis of sulfaquinoxaline byproducts formed in water upon solar light irradiation. Sulfonamides such as sulfaquinoxaline (SQX) are among the most important antibiotic families due to their extensive use in veterinary medicine. The prediction of their fate under solar irradiation through the identification of the generated metabo...

Sahai, O.K.uth, M.P.rk, G.N.eder,, H., 1986: Producing high value food ingredients via plant biotechnology. World biotech report 1986: proceedings of Biotech 86 Europe held in London May 1986: 6

Bopp, M., 1974: Action mechanism of cytokinins in mosses (a model for the effects of cytokinins). Plant Growth Substances Proceedings of the International Conference: ub 1974), 8th 934-944