geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Continuous assay of serum 5' nucleotidase ec 3.1.3.5 activity with imp as substrate and total automation using a transfer analyzer kem o mat


, : Continuous assay of serum 5' nucleotidase ec 3.1.3.5 activity with imp as substrate and total automation using a transfer analyzer kem o mat. Journal of Clinical Chemistry and Clinical Biochemistry 21(8): 481-490

The continuous spectrophotometric assay of 5'-nucleotidase originally described by Heinz et al. (1980) was modified and fully automated on a Kem-O-Mat transfer analyzer, using IMP as substrate. The reaction product was H2O2 and the reduction of NADP was observed for 10 min at 340 nm and at a reaction temperature of 30.degree. C. The different factors involved in the enzyme reaction were checked, including the substrate concentration, reaction rate, linearity and substrate preservation. Normal values ranged from 1-13 U/l. Between-day reproducibility was estimated with 2 different commercial control sera, and the coefficient of variation was 5% for the upper limit of normal activity (23 U/l). There was good agreement between the present method and a semi-automatic colorimetric technique (for 100 [human] sera tested by both methods, r = 0.974 and the regression line equation, y = 0.85x - 1.5). Despite the lengthy reagent mixture preparation procedure, the method permitted assay of 50 samples/h. The occurrence of high serum blanks in certain pathological states is discussed.

US$29.90


Other references

Malling H.V., 1976: Comparison between in vitro and in vivo activation. De Serres, Frederick J Et Al (Ed ) in Vitro Metabolic Activation in Mutagenesis Testing Proceedings Of The Symposium on The Role Of Metabolic Activation in Producing Mutagenic And Carcinogenic Environmental Chemicals Research Triangle Park, N C , Usa, Feb 9-11, Vii+363p Illus Elsevier/North-Holland Biomedical Press: Amsterdam, Netherlands; Elsevier/North-Holland, Inc : New York, N Y , Usa Isbn 0-7204-0612-9 143-156

Atale, S.B.; Vitkare, D.G.; Gharde, G.N., 1996: Stability analysis in maize (Zea mays L.) for grain yield and its component traits. Information on genotype environment interaction is derived from data on flag leaf area, plant height, days to maturity, grains/cob and grain yield/plant in 14 maize genotypes (8 hybrids and 6 composites) grown in 8 environments at Akola during kha...

Yokoyama, I.; Hayakawa, A.; Hayashi, S.; Negita, M.; Katayama, A.; Uchida, K.; Takagi, H., 1995: Apoptosis induction in isolated mouse hepatocytes by immunosuppressants in vitro. Hepatology 22(4 PART 2): 229A

Baumann, G.Ace, J.B.; Tomcsik, J., 1956: Effect of mechanical action on cell walls of gram-positive bacteria. Schweizerische Zeitschrift für Pathologie und Bakteriologie. Revue Suisse de Pathologie et de Bacteriologie 19(5): 566-575

Bernard, J.; Beignot-Devalmont, M.; Sebastien, F.; Pouillot, M.J.; Desvignes, A., 1980: Comparative study of the in vitro- and vivo- antimicrobial activity of seven antiseptic solutions for use on surgeon's hands. The antibacterial activity of 7 antiseptic solutions has been studied both "in vitro en in vivo". The results obtained in both case were necessary and complementary since some products (e. g. Solubacter) show an irregular activity either...

Endres, M.; Ahmadi, M.; Kruman, I.; Biniszkiewicz, D.; Meisel, A.; Gertz, K., 2004: Folate deficiency increases postischemic brain injury. Background and Purpose - Folate deficiency and resultant hyperhomocysteinemia impair vascular function and increase stroke risk. We tested the hypothesis that folate deficiency and high homocysteine levels promote DNA damage and increase brain inj...

W.S.Y.; Colombetti L.G., 1988: Iodine 131 metaiodobenzylguanidine mibg and extra vesicular accumulation in rat brown adipose tissue. European Journal of Nuclear Medicine 14(5-6): 306

Colford J.; Smith A.; Parker R., 1990: Dietary selenite enhances afb 1 detoxification and reduces af dna binding in isolated rat hepatocytes. FASEB Journal 4(4): A1175

Chen, H.; Kim, S.; He, W.; Wang, H.; Low, P.S.; Park, K.; Cheng, J-Xin., 2008: Fast release of lipophilic agents from circulating PEG-PDLLA micelles revealed by in vivo forster resonance energy transfer imaging. Understanding the in vivo behavior of nanoparticles is critical for the translation of nanomedicine from laboratory research to clinical trials. In this work, in vivo Forster resonance energy transfer (FRET) imaging was employed to monitor the rel...

van der Kooij, Henk., 1998: Are the breeding results of purple herons Ardea purpurea influenced by change of nest height? Wordt het broedresultaat van purperreigers Ardea purpurea beinvloed door de nesthoogte?. Limosa. 1997( ); 704: 145-150