+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Core particles of hepatitis b virus and ground squirrel hepatitis virus 2. characterization of the protein kinase reaction associated with ground squirrel hepatitis virus and hepatitis b virus

, : Core particles of hepatitis b virus and ground squirrel hepatitis virus 2. characterization of the protein kinase reaction associated with ground squirrel hepatitis virus and hepatitis b virus. Journal of Virology 43(2): 741-748

The recently described protein kinase activity in hepatitis B virus core antigen particles has been demonstrated in the liver-derived core particles of ground squirrel hepatitis virus. Both protein kinase activities were initially associated with DNA polymerase-positive heavy core particles in CsCl density equilibrium gradients and shifted to polymerase-negative cores during the course of purification. The major core-associated polypeptide of each virus was the dominant species labeled. A variable number of other polypeptide species were also labeled by this reaction. Tryptic peptide mapping of both major and minor phosphorylated polypeptides of each virus resulted in similar patterns, suggesting that many of the sites of phosphorylation were the same in the components of each core particle. Hydrolysis of these phosphorylated core particles revealed a major phosphoamino acid as serine and a minor phosphoamino acid as threonine. The products of the protein kinase reaction in both human hepatitis B and ground squirrel hepatitis virus core particles, then, share many characteristics. The possible function(s) of this protein kinase activity is discussed in the light of similarly characterized activities in other animal viruses.


PMID: 7109041

Other references

Linnett P.E.; Mulheirn L.J.; Beechey R.B., 1983: The stoichiometry of binding of the aurovertins to mitochondrial atpase revision of the molar absorption coefficient. The purity of aurovertins A, B and D, which inhibit the mitochondrial ATPase and show fluorescence enhancement when bound, was determined by high-resolution 1H NMR. This technique demonstrated that solvent molecules, especially water, are tightly...

Dearry, M.S.; Long, E.C., 2001: DNA recognition by Ni Xaa-Gly-His metallopeptides containing modified proline residues. Abstracts of Papers American Chemical Society 222(1-2): INOR157

Koyanagi, J., 1977: Combined valvular diseases. Nihon Kyobu Geka Gakkai 25(5): 554-556

Monteith, S.James.; Yen, C-Po.; Sheehan, J.P., 2011: Gamma knife surgery for pediatric arteriovenous malformations: a review. Clinical Neurosurgery 58: 126-132

Campbell, S.; Mchaffie, D.; Whaley, S.; Stone, W.; Krishnan, K., 2003: Differences in the ability of alpha-tocopherol and gamma-tocopherol to suppress deoxycholic acid -induced suppression of cyclooxygenase-2 protein in HCT-116 and HT-29 human colon cancer cell lines. Cancer Epidemiology Biomarkers & Prevention 12(11 Part 2): 1296s, November

Rathman R.J.; Brunner J.F.; Hulbert S.J., 1988: Feeding by medetera spp diptera dolichopodidae on aphids and eriophyid mites on apple malus domestica rosaceae. Proceedings of the Entomological Society of Washington 90(4): 510-512

Kolenović, E., 2005: Correlation between intensity and phase in monochromatic light. Analytical expressions to describe the phase gradient of monochromatic light by means of the three-dimensional intensity distribution are derived. With these formulas it is shown that the two-dimensional phase gradient in a plane can be completely...

Tsoïtis, G.; Kanitakis, C.; Kapetis, E., 1983: Multinodular plaque-type blue nevus, superficial and neuroid. We report an exceptional case of multinodular plaque-type blue nevus in a 63-year-old woman, located on the presternal region. The lesions appeared at the age of 25 years on an achromic area. The probable clinical diagnosis was that of a pigmentar...

Horigome, A.; Nagasawa, N.; Ikeda, K.; Ito, M.; Itoh, J-Ichi.; Nagato, Y., 2009: Rice open beak is a negative regulator of class 1 knox genes and a positive regulator of class B floral homeotic gene. Numerous genes are involved in the regulation of plant development, including those that regulate floral homeotic genes, We identified two recessive allelic rice mutants, open beak-1 (opb-1) and opb-2, which exhibited pleiotropic defects in leaf m...

Sun Wenyue, 1998: The regularity of structural ore controls and their significance at the Tongshan copper deposits, Yangtze Valley, China. The Tongshan copper deposit is located in the Fe-Cu metallogenic belt of the famous middle-lower Yangtze valley. It is a middle-sized skarn copper deposit. This paper places emphasis on the regularity of the structural ore control for this deposit...