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Correlation between transcription intensity and the content of ribosomal rna genes in individual nucleolar forming regions of human chromosomes


, : Correlation between transcription intensity and the content of ribosomal rna genes in individual nucleolar forming regions of human chromosomes. Byulleten' Eksperimental'noi Biologii i Meditsiny 105(1): 63-65

A comparison has been carried out of satellite strand lengths, Ag-staining intensity and labelling intensity in in situ hybridization of 3H-thymidine-labelled 28S-rRNA gene fragment in nucleolar-forming regions (NFR's) of individual acrocentric chromosomes from blood lymphocytes of 2 caryotypically normal individuals. To identify chromosomes modified R-staining was performed (5-bromodesoxypyridine + Hoechst fluorochrome 33258 + Gimsa dye). The data obtained demonstrate, firstly, the variability between 10 acrocentric human chromosomes both in the content of ribosome genes and in silvering intensity and NFR satellite strand length and, secondly, a positive correlation between three studied characteristics of individual chromosomes. In one case an exception has been noted in one homologue of chromosome 21 of an individual A: high intensity of hybridization labelling was accompanied by weak Ag-staining and short satellite strand. It was concluded that the variability of transcription activity of individual NFR's detected by Ag-staining is, as a rule, based on the variability in the content of ribosome gene DNA in them and, in some cases, the presence of rRNA gene copies in inactive state.

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