geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Correlation of immunogenicity and production of ornithine by peritoneal macrophages


, : Correlation of immunogenicity and production of ornithine by peritoneal macrophages. Journal of Immunology 139(3): 899-904

The release of ornithine by macrophages and its correlation with their immunogenicity after treatment with various macrophage-stimulating substances were analyzed. Pristane-elicited peritoneal macrophages (PM) were found to express strong arginase activity and to release L-ornithine into the extracellular space. This activity is strongly reduced within 3 hr after treatment with tetradecanoylphorbol acetate (TPA) but not with lipopolysaccharide (LPS). Resident PM usually express little arginase activity, but this activity is markedly augmented within 24 or 48 hr after treatment with LPS. The release of ornithine by peritoneal cells (PC) (60 to 90% macrophages) was found to be correlated with their immunogenicity as determined by the in vivo immunization for a subsequent in vitro secondary cytotoxic response against minor H antigens. The immunogenicity of pristane-elicited PC is markedly stronger than that of resident PC or TPA-treated, pristane-elicited PC. Moreover, the immunogenicity of the resident PC and TPA-treated elicited PC is substantially augmented by the simultaneous injection of ornithine, whereas the immunogenicity of the untreated elicited PC is not further augmented by exogenous ornithine, indicating that the endogenous production of ornithine by the stimulating cells has a strong influence on the resulting immune response. Injection of glutathione into pristane-treated mice also reduces the ornithine production and immunogenicity of the resulting peritoneal exudate cells. The immunogenicity in this case is at lest partly reconstituted by application of exogenous ornithine. Our experiments revealed no correlation between the production of ornithine and prostaglandin E2. Prostaglandin E2 production of resident and pristane-elicited PC is not markedly different and is in either case strongly augmented by TPA. Elicited or resident PM which have been incubated fore several days in cultural release practically no ornithine; but ornithine production can be induced again by incubation for 24 hr with LPS and to some extent also with interferon-.gamma.

US$29.90

PMID: 3110288


Other references

Richardson, R.; Riccio, D.C.; Jonke, T., 1983: Alleviation of infantile amnesia in rats by means of a pharmacological contextual state. Although a number of studies have demonstrated that the relatively rapid rate of forgetting in young rats (i.e., infantile amnesia) can be alleviated by various reminder treatments, the possibility of enhancing retention by other procedures seems...

Flint, A.C.; Anziska, Y.; Rausch, M.E.; Herzog, T.J.; Williams, O., 2006: A clinical and radiographic variant of Wernicke-Korsakoff syndrome in a nonalcoholic patient. Neurology 67(11): 2015-2015

Wolff, George L., 2000: 1905-2000 Regulation of phaeomelanogenesis in yellow agouti mice. Pigment Cell Research 13(3): 198-199, June

Baruch, M., 2001: Aversion of doctors to the subject of marketing. Klinische Monatsblatter für Augenheilkunde 218(4): A74-A75

Fleenor, D.E.; Langdon, S.D.; D.C.stro, C.M.; Kaufman, R.E., 1996: Comparison of human and Xenopus GATA-2 promoters. GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identif...

Krause, T.Millard.; Ukhanova, M.; Revere, F.Lee., 2016: Private Carriers' Physician Payment Rates Compared With Medicare and Medicaid. This research evaluated the 2013 published physician reimbursement rates for Medicare and Medicaid in Texas and compared the rates with the mean fees from private carriers. Physician claims data were extracted from the Truven MarketScan Commercial...

Cutz, E.; Viero, S.; Campbell, D.M.; Satodia, P.; Parvez, B., 2004: Congenital acinardysplasia Spectrum of pathology. Modern Pathology 17(2): 267, February

Ruiz, L.; Romeu, J.; Martinez Picado, J.; Schmidt, J.C.aude; Vandamme, A.M.eke; Balague, M.; Cabrera, C.; Piug, T.; Tural, C.; Segura, A.; Sierra, G.; D.C.ercq, E.; Clotet, B., 1996: Efficacy of triple combination therapy with zidovudine plus zalcitabine plus lamivudine versus double combination therapy in patients previously treated with ZDV + ddC. Objective: To evaluate the immunological and virological efficacy of triple combination therapy with zidovudine (ZDV) plus zalcitabine (ddC) plus lamivudine (3TC) and a double (ZDV+3TC) combination therapy in patients previously treated with ZDV p...

Shrawder E.J.; Foster G., 1981: Enzyme immunoassay for retinol binding protein and prealbumin in plasma. Journal of Clinical Chemistry & Clinical Biochemistry 19(8): 837

Okame, R.; Nakahara, K.; Kato, Y.; Bannai, M.; Murakami, N., 2016: Comparison of amino acid profiles between rats subjected to forced running and voluntary running exercises. It has been suspected that in comparison with glucose or fatty acids, the levels of amino acids may readily change with different forms of exercise. In the present study, we measured the concentrations of amino acids, glucose, triglycerides, total...