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Deoxyribose ring conformation of d ggtatacc2 an analysis of vicinal proton proton coupling constants from two dimensional proton nmr

, : Deoxyribose ring conformation of d ggtatacc2 an analysis of vicinal proton proton coupling constants from two dimensional proton nmr. Biochemistry 27(16): 6013-6020

Exchangeable and nonexchangeable protons of [d(GGTATACC)]2 in aqueous cacodylate solution were assigned from two-dimensional nuclear Overhausser effect (2D NOE) spectra. With phase-sensitive COSY and double quantum filtered COSY (DQF-COSY) experiments, the cross-peaks resulting from deoxyribose ring conformation sensitive proton-proton vicinal couplings, i.e., all 1'-2', 1'-2", 2'-3', and 3'-4' couplings and six from 2"-3' couplings, were observed. From the cross-peak find structure, the 2',2" proton assignments can be confirmed; coupling constants J1'2' and J1'2" and sums of coupling constants involving H2' and H2" for all residues and H3' for C8 were obtained. The DISCO procedure [Kessler, H., Muller, A., and Oschkinat, H. (1985) Magn. Reson. Chem. 23, 844-852] was used to extract individual 1'-2' and 1'-2" coupling constants. The sum of coupling constants involving H1' or H3' was measured from the one-dimensional spectrum where signal overlap is not a problem. Analysis of the resulting coupling constants and sums of coupling constants, in the manner of Rinkel and Altona [Rinkel, L. J., and Altona, C. (1987) J. Biomol. Struct. Dyn. 4, 621-649], led to the following conclusion: C2'-endo deoxyribose ring conformation is predominant for every residue, but a significant amount of C3'-endo conformation may exist, ranging from 14% to 30%.


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Manai, H.; Paulet, P., 1973: Variations in the rutin content of Nicotiana sylvestris and Nicotiana tabacum cultivar Maryland Mammoth as affected by photoperiod. In tobacco cv. Maryland Mammoth, a short-day species. the rutin content of leaves grown under 9-h photoperiods was 0.08% in basal leaves, 0.14% in leaves from the middle of the stem and 0.25% in upper leaves, and the rutin content of the stem incr...

Randall Allan D.; Francis Rory M.; Frimpter Michael H.; Emery James M., 1988: Region 19, Northeastern Appalachians. O 2; Pages 177-187:

Totonchy, J.; Cesarman, E., 2016: Does persistent HIV replication explain continued lymphoma incidence in the era of effective antiretroviral therapy?. NlmCategory="UNASSIGNED">Non-Hodgkin lymphomas are highly increased in incidence in individuals infected with HIV, and this continues to be the case in spite of highly effective combined antiretroviral therapy (cART). New evidence has...

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Montie, J.E.; Severson, R.K.; Porter, A.T.; Demers, R.Y., 1995: Observations on recent trends in the treatment of prostate cancer amongst elderly men. Journal of Urology 153(4 SUPPL ): 390A

Zubiri, I.; Posada-Ayala, M.; Sanz-Maroto, A.; Calvo, E.; Martin-Lorenzo, M.; Gonzalez-Calero, L.; de la Cuesta, F.; Lopez, J.A.; Fernandez-Fernandez, B.; Ortiz, A.; Vivanco, F.; Alvarez-Llamas, G., 2014: Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis. Diabetic nephropathy (DN) is a major complication of diabetes mellitus (DM), the most frequent cause of end-stage renal disease (ESRD). Exosomes isolated from urine are considered a rich non-invasive source of markers for renal events. Proteinuria...

Hong, L.Elliot.; Summerfelt, A.; Wonodi, I.; Adami, H.; Buchanan, R.W.; Thaker, G.K., 2007: Independent domains of inhibitory gating in schizophrenia and the effect of stimulus interval. Objective: Patients with schizophrenia are known to have inhibitory gating deficits in the suppression of evoked potential P50 response to repeated stimuli and the prepulse inhibition of the startle response. In the current study, the authors aim...

Baumgartner, C.; Brun del Re, G.P.; Bleher, E.A.; Bucher, U.; Delaleu, B.; Frei, H.; Greiner, R.; Hirt, A.; Imbach, P.; Lüthy, A., 1985: Autologous bone marrow transplantation--clinical experience in Berne. Autologous bone marrow transplantation was performed in 28 pediatric and adult patients with various neoplasias. Long-term remissions were obtained in one patient with yolk sac tumor and in 9 patients with B-cell non-Hodgkin's lymphoma. The r...

Friedel M.J.; Schmidt R.D., 1992: Effect of unsaturated conditions on the hydrology of in situ copper leaching. Mining Engineering 44(8): 1029-1036

Astre, G., 1936: Ossements d'un tumulus a Lassalle (Hautes-Pyrenees). Bull J Soc Hist nat Toulouse, 70: 349-350