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Detachment of cells in vitro with culture fluids from stimulated human mononuclear cells

, : Detachment of cells in vitro with culture fluids from stimulated human mononuclear cells. Journal of Periodontal Research 13(4): 372-381

Supernatants of cultured mononuclear cells (S-MNL) were tested for their ability to separate cells from their substrates. Adherent [mouse fibroblasts] L-cells were exposed for up to 3 h to culture fluids from MNL [mononuclear cells] with and without PHA [phytohemagglutinin] stimulation. A reproducible shearing force was applied to the L-cells to test for loss of cohesiveness at the cell periphery. A significant increase of 3-fold or greater was found in the number of detached L-cells receiving stimulated S-MNL when compared with culture fluids from unstimulated MNL. The detachment-enhancing activity was present in culture fluids within 24 h following stimulation. MNL culture fluids revealed no differences in the quantities of lysosomal enzymes to explain the increased cell detachment. The detachment of cells seen in vitro by products released from mononuclear cells may provide an explanation for the in vivo ability of these cells to infiltrate tissue.


PMID: 149857

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