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Determination of amide nitrogen in plant proteins using an ammonia electrode

, : Determination of amide nitrogen in plant proteins using an ammonia electrode. Canadian Institute of Food Science & Technology Journal 14(3): 227-229

The use of an ammonia electrode for determination of ammonia released from asparagine and glutamine residues during mild acid hydrolysis was investigated. Comparison of this technique to a Conway microdiffusion method and a technique employing an amino acid analyzer gave values which were not significantly different (P = 0.05) from those produced with the amino acid analyzer. Both gave results higher than those obtained with Conway microdiffusion. The precision of the electrode technique was good with a coefficient of variation of 4.5%. The ease of operation and reduced time for analysis make the ammonia electrode technique most useful in quantitating amidated glutamic and aspartic residues.


DOI: 10.1016/S0315-5463(81)72876-1

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