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Determination of enantiomers of amino acids by reversed phase high performance liquid chromatography

, : Determination of enantiomers of amino acids by reversed phase high performance liquid chromatography. Analytical Chemistry 52(8): 1206-1209

The chromatographic resolution of enantiomeric amino acids is accomplished on reversed phase columns using aqueous mobile phases containing the chiral reagent L-aspartylcyclohexylamide-Cu(II) This reagent has the additional advantage that the Cu ions allow the detection of nonaromatic amino acids at 230 nm. The separation seems to be a result of hydrophobic interactions between the cyclohexylamide moiety of the chiral additive and the side chain of the amino acids.


DOI: 10.1021/ac50058a011

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