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Development of preliminary application of a homologous radio immunoassay for bull frog prolactin


, : Development of preliminary application of a homologous radio immunoassay for bull frog prolactin. General and Comparative Endocrinology 32(4): 531-535

A homologous radioimmunoassay (RIA) with high sensitivity was developed for bullfrog prolactin (fPRL). The fPRL used for immunization, iodination and reference preparation was obtained by disc gel electrophoresis of frog anterior pituitaries. Bullfrog growth hormone (fGH) showed minimal cross-reactivity, and plasma from hypophysectomized frogs had no detectable immunoreactive PRL. The antiserum to fPRL inactivated biologically active PRL in tadpoles and caused accelerated metamorphosis. The levels of immunoreactive fPRL were determined in individual whole pituitaries and in pooled plasma samples from Rana catesbeiana tadpoles through metamorphic climax (Taylor-Kollros stages XXI-XXV). Pituitary PRL rose slowly from the emergence of the forelegs through stage XXIII and then increased markedly in stage XXIV. Circulating levels paralled pituitary content, except that the rise during stage XXIV was less pronounced.

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