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Differences in the buoyant density of mitochondria isolated from hepatoma krebs ii ascites tumor and normal liver cells


, : Differences in the buoyant density of mitochondria isolated from hepatoma krebs ii ascites tumor and normal liver cells. Voprosy Onkologii (Stsburg). 1975; 21(5): 74-79

Mitochondria isolated from normal liver, experimental hepatoma and Krebs II ascites tumor tissues in mice were examined by isopycnic centrifugation in sucrose density gradient (25-50%). Normal liver mitochondria were characterized by 2 visible zones, while those of tumors were distributed homogeneously. The distribution pattern was also confirmed by the investigation of marker enzyme activities (glucose-6-phosphatase and cytochromoxidase). A comparison of homologous tissues (hepatoma liver) showed that hepatoma mitochondria were lighter than the light fraction of normal ones which may evidence the disturbances in the biogenesis of these organelles.

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