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Differentiation of rhizobium leguminosarum strains by ir spectroscopy


, : Differentiation of rhizobium leguminosarum strains by ir spectroscopy. Biology Bulletin of the Academy of Sciences of the USSR 9(6): 565-569

A differentiation of R. leguminosarum strains was carried out under conditions of pure culture on the basis of IR spectral analysis of whole microbial cells. The criterion of differentiation was the ratio of absorption band intensity, detected by the presence of polyhydroxybutyric acid (PHB) in the cells, to an absorption band intensity in the 1660 cm-1 range, detected mainly by proteins. R. leguminosarum strains differ in their ability toward intracellular accumulation of PHB on sucrose and aminopeptide medium, while the greatest amount of the polymer was accumulated on the given medium by the nonactive strain. Conditions of microbial culture growth are responsible for the taxonomic value of IR spectra of whole cells.

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