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Effect of cytosol on transport of RNA in vitro during cardiac hypertrophy

, : Effect of cytosol on transport of RNA in vitro during cardiac hypertrophy. Biochemical Journal 209(2): 285-290

An enhanced RNA-transport activity was observed in vitro from nuclei obtained from animals [rats] with cardiac hypertrophy as compared with that of sham-operated controls. The 100,000 g supernatants obtained from hypertrophic hearts stimulated the RNA transport from nuclei of sham-operated controls, and this stimulation was maximum with 40% supernatant. Ca2+- and nucleic acid-dependent ATPase and alkaline phosphatase activities, which may be involved in an energy-dependent transport, were high in nuclei from hypertrophic hearts, and the nuclei of sham-operated animals showed higher activities of these enzymes after incubation with supernatant from hypertrophic hearts, which stimulates the RNA transport in vitro from nuclei of sham-operated animals.


PMID: 6189481

DOI: 10.1042/bj2090285

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