+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of ATP on lysosomes: inhibition of the loss of latency caused by cooling

, : Effects of ATP on lysosomes: inhibition of the loss of latency caused by cooling. American Journal of Physiology 242(3): C192-C199

Triton-filled lysosomes from rat liver and a crude lysosomal fraction from livers of rats not injected with Triton have been used to study the ability of ATP to protect lysosomes against the effects of incubation in vitro at 37 degrees C. When incubation was carried out in a sucrose-NH4Cl medium at pH 8.0 or in buffer containing 0.15 M KCl at pH 7.4, ATP protected against the loss of lysosomal enzyme latency that occurred at 37 degrees C and also against the additional loss of latency that occurred when the incubated lysosomes were cooled to 0 degrees C. The inhibition of the effects of cooling was found to result from a decrease in the loss of latency caused specifically by cooling the lysosomes through the temperature range of the membrane phase transition (15-0 degrees C); ATP did not affect the basic rate of loss of latency at 0 degrees C. Protection was minimal when incubation was carried out in 1 mM ethylenediaminetetraacetic acid without added divalent metal ion and was enhanced by the addition of either Mg2+ or Ca2+. Kinetic studies were carried out on the ATP-protective action against the effects of cooling by adding ATP to lysosomes that had been incubated at 37 degrees C and that were then cooled to 0 degrees C at various times after addition of the ATP. Susceptibility to the effects of cooling continued to decrease until the ATP had been present at 37 degrees C for approximately 3 min before cooling. The concentration of ATP required for maximum initial rates of protection was found to be 10(-3) M or possibly lower. The rate of decrease in susceptibility to the effects of cooling was much slower when ATP was added at 22 than at 37 degrees C, suggesting that enzyme action may be involved in the protective mechanism. Possible mechanisms for the ATP-protective action are discussed.


PMID: 7065169

Other references

Brobeck, J.R., 2014: Regulation of energy exchange in rats. Federation Proceedings 6(1 Pt 2): 82-82

Memon, G.N.; Patel, B.M.; Buch, N.C., 1971: Effect of dietary energy from hydrogenated vegetable oil and starch on ovarian and anterior pituitary gland response in ewes. For 75 Targhee ewes of 61.3 kg average bodyweight, in 5 groups, pelleted rations given for 51 days supplied digestible energy 3.3 Mcal daily, for maintenance, or 4-3 or 5.3 Mcal, each supplied by extra fat or starch. Ovulation rates were 1.33 and...

Ghante, M.H.; Bhusari, K.P.; Duragkar, N.J.; Jain, N.S.; Warokar, A.S., 2012: Bronchorelaxant, mast cell stabilizing, anti-inflammatory and antioxidant activity of Randia dumetorum (Retz.) Lamk. extracts. Randia dumetorum (RD) fruits in different form have been ethnopharmacologically reported to possess antiasthmatic property. Therefore, present study was undertaken to evaluate two different extracts of RD i.e., ethyl acetate (RD-EA) and methanol (...

Anonymous, 1999: Managed care woes: industry trends and conflicts. Although managed care plans have had success in controlling costs, they now face challenges on many fronts, including tighter profit margins, pressure for broader provider networks, increasing clout of hospitals and physicians and more demand for...

Tribe, R.H.; Sendt, K-Verena.; Tracy, D.K., 2017: A systematic review of psychosocial interventions for adult refugees and asylum seekers. Europe is in the midst of the largest refugee migration since the Second World War; there is an urgent need to provide an updated systematic review of the current best evidence for managing mental distress in refugee populations. The aim of this r...

McLean Hodgson J.; Kempton N.H., 1979: The Oakey-Dalby coalfield stratigraphy and depositional environments. High ash but ammenable to beneficiation.

Nickell, L.G., 1964: Tissue and cell cultures of sugarcane . . . another research tool. These cultures provide an economical method for the following types of investigation: conventional and radioactive-isotope nutritional studies; growth and. growth rates; mutation; phytotoxicity; response to irradiation; transformation of plant typ...

D.L.u, H.M.; Hutter, J.C., 2000: Physiologically based pharmacokinetic model for octamethylcyclotetrasiloxane. Abstracts of Papers American Chemical Society 219(1-2): TOXI 55

Truckai, C., 1995: Shapable handle for steerable electrode catheter. Official Gazette of the United States Patent & Trademark Office Patents 1172(2): 986, March 14

Vender, S.; Poloni, N., 2007: Is the insight a favourable prognostic factor in the treatment of mental disorders?. In the history of psychology, psychoanalysis and psychiatry, the concept of insight has had alternate fortunes and has been variously interpreted according to the observation apex from which it has been studied. In psychiatry, particularly, the in...