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Effects of bacillus thuringiensis nuclear polyhedrosis virus mixtures on trichoplusia ni larvae


, : Effects of bacillus thuringiensis nuclear polyhedrosis virus mixtures on trichoplusia ni larvae. Journal of Invertebrate Pathology 29(3): 367-372

Effects of exposure to B. thuringiensis and a nuclear-polyhedrosis virus (NPV) on larvae of the cabbage looper, T. ni, were determined in laboratory experiments. Mortality curves were established for each pathogen and served as the basis for the tests. Third-instar larvae averaging 15 mg body wt were inoculated by being on a diet containing desired concentrations of the pathogens. The ELD50 [median effective lethal dose] for B. thuringiensis was 300 IU/ml of diet and for NPV, 2.27 .times. 103 polyhedral inclusion bodies/ml of diet. Mortality data from larvae exposed to both pathogens simultaneously at generally low dosages indicated that the effects of the pathogens in combination were additive. Results from experiments in which larvae were stressed by exposure to NPV for 24-96 h prior to exposure to B. thuringiensis showed that increased mortality was an additive effect of the 2 pathogens. Pupae from larvae exposed to B. thuringiensis were significantly smaller than those from larvae exposed to NPV alone or to no pathogen.

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