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Effects of nitrogen starvation on the function and organization of the photosynthetic membranes in Cryptomonas maculata (Cryptophyceae)

, : Effects of nitrogen starvation on the function and organization of the photosynthetic membranes in Cryptomonas maculata (Cryptophyceae). Planta 169(3): 361-369

Nitrogen deficiency affects both photosystems and the antennae pigment systems in the photosynthetic apparatus of the marine alga, Cryptomonas maculata. Under increasing energy fluence rates, O2 evolution in nitrogen-deficient (-N) cell suspensions never reached a positive value; in control cultures (+N), O2 evolution increased and was saturated at about 6.4 W .cntdot. m-2 with about 100 .mu.mol chlorophyll-1.cntdot.h-1. During fluorescence-induction experiment at room temperature, F0 and Fmax were significantly increased in -N cells whereas the Fvar/Fmax ratio decreased from 0.6 to 0.1. These observations can be correlated with a significantly decreased population of 12.5-nm-size particles in the exoplasmic-fracture (EF) faces of freeze-cleaved thylakoid membranes in -N cells (Rhiel et al., 1985, Protoplasm 129, 62-73). The EF particles are suggested to represent photosystem II associated with chlorophyll a/c-protein complexes (LHCP). The banding pattern of isolated and Triton X-100-solubilized thylakoid membranes of both +N and -N cells in sucrose gradient showed that the LHCP is still present in -N cells. The same applies to sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these membrane fractions. The reduced number of the 12.5-nm particles in the EF faces of -N cells may be a result of decoupling of the LHCP constituents of the photosystem-II complex rather than their degradation. This is supported by high values for the initial fluorescence Fo in fluorescence-induction experiments, and in part, is indicated by the shift of the maximal fluorescence emission from 693 nm in +N to 684 nm in -N cells. The lack of the CP1 band in the gels of sodium dodecyl sulfate-solubilized thylakoid membranes from -N cells after electrophoresis demonstrates that photosystem I is also severely affected.


PMID: 24232648

DOI: 10.1007/BF00392132

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