geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of nitrogen starvation on the function and organization of the photosynthetic membranes in Cryptomonas maculata (Cryptophyceae)


, : Effects of nitrogen starvation on the function and organization of the photosynthetic membranes in Cryptomonas maculata (Cryptophyceae). Planta 169(3): 361-369

Nitrogen deficiency affects both photosystems and the antennae pigment systems in the photosynthetic apparatus of the marine alga, Cryptomonas maculata. Under increasing energy fluence rates, O2 evolution in nitrogen-deficient (-N) cell suspensions never reached a positive value; in control cultures (+N), O2 evolution increased and was saturated at about 6.4 W .cntdot. m-2 with about 100 .mu.mol O2.cntdot.mg chlorophyll-1.cntdot.h-1. During fluorescence-induction experiment at room temperature, F0 and Fmax were significantly increased in -N cells whereas the Fvar/Fmax ratio decreased from 0.6 to 0.1. These observations can be correlated with a significantly decreased population of 12.5-nm-size particles in the exoplasmic-fracture (EF) faces of freeze-cleaved thylakoid membranes in -N cells (Rhiel et al., 1985, Protoplasm 129, 62-73). The EF particles are suggested to represent photosystem II associated with chlorophyll a/c-protein complexes (LHCP). The banding pattern of isolated and Triton X-100-solubilized thylakoid membranes of both +N and -N cells in sucrose gradient showed that the LHCP is still present in -N cells. The same applies to sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these membrane fractions. The reduced number of the 12.5-nm particles in the EF faces of -N cells may be a result of decoupling of the LHCP constituents of the photosystem-II complex rather than their degradation. This is supported by high values for the initial fluorescence Fo in fluorescence-induction experiments, and in part, is indicated by the shift of the maximal fluorescence emission from 693 nm in +N to 684 nm in -N cells. The lack of the CP1 band in the gels of sodium dodecyl sulfate-solubilized thylakoid membranes from -N cells after electrophoresis demonstrates that photosystem I is also severely affected.

US$19.90

PMID: 24232648

DOI: 10.1007/BF00392132


Other references

Jeong, K.-Heon.; Lee, Y.-Seok.; Shim, Y.-Bo., 1999: Cytochemical and immunocytochemical study on the cellulase activity in the accessory glands of the digestive system of the oriental land snail, Nesiohelix samarangae. Korean Journal of Malacology. December; 152: 81-92

Sham, J.S.K.; Song, L.S.; Stern, M.D.; Lakatta, E.G.; Cheng, H., 1999: Inactivation of cardiac ryanodine receptor Evidence from restitution of Ca2+ release. Biophysical Journal 76(1 PART 2): A385, Jan

Erickson, 2000: Dual-purpose ion source. Analytical Chemistry 72(17): 578a-579a

Chawla, B.; Luxton-Andrew, H., 2008: Long-term weight loss observed with olanzapine orally disintegrating tablets in overweight patients with chronic schizophrenia. A 1 year open-label, prospective trial. Objective To investigate the long-term weight loss outcomes during usual clinical practice after switching from olanzapine standard oral tablet (SOT) to olanzapine orally disintegrating tablets (ODT).Method In this open-label prospective study, 2...

Williams, R.C., 1959: Dermatomyositis and malignancy: a review of the literature. The first report of dermato-myositis associated with malignancy in 1916 by Stertz has been followed by increasing attention in the literature. Of 590 cases reported in the literature, 92 have had tumor accompanying dermatomyositis. In these 92 cas...

Kristensen, P.Lommer.; Petersen, Ré.Horsleben.; Hansen, P.Boye., 2007: Spontaneous hemothorax from a fibrous pleural tumour in an expectant father. Is the delivery room a dangerous place for men?. A 34-year-old male was admitted to our department with dyspnoea and severe pain in the left hemithorax. The symptoms had started suddenly three days before in the delivery room when his child was being born. An X-ray of the chest showed a tumour i...

Gaur, S.N.; Gangwar, M.; Khan, Z.U.; Jain, S.K.; Randhawa, H.S., 1992: Farmer's lung disease in north-western India--a preliminary report. A study of farmer's lung (FL) disease was carried out in 197 subjects engaged in farming and having respiratory complaints of varying duration. It revealed that 13.2% of the subjects had precipitating antibodies against thermophilic actinomyc...

Ling, S.M.; Morris, C.; Fried, L.; Hirsch, R.; Hochberg, M., 1997: Accuracy of self-reported physician diagnosis of rheumatoid arthritis in a cohort of disabled elderly women. Arthritis & Rheumatism 40(9 SUPPL ): S113, Sept

Buck W.R.ger; Poliakov Alexei N.; Lavier Luc L., 1998: Controls of normal faulting and abyssal hill formation at mid-ocean ridges. Eos, Transactions, American Geophysical Union 79(45, Suppl

Prior, J.C.; Wark, J.D.; Barr, S.I., 1993: The prevention and treatment of osteoporosis. New England Journal of Medicine 328(1): 65; Author Reply 66-65; Author Reply 66