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Enzyme linked immuno sorbent assay for leucine enkephalin and methionine enkephalin

, : Enzyme linked immuno sorbent assay for leucine enkephalin and methionine enkephalin. Analytical Biochemistry 130(1): 83-87

An enzyme-linked immunosorbent assay for enkephalins was develped by coupling the peptides to a carrier molecule (bovine serum albumin) in order to allow the antibody-antigen reaction to take place in the solid phase. The assay was highly reproducible. Its sensitivity was 14 nmol/l for leucine enkephalin and 27 nmol/l for methionine ekephalin, which is similar to that obtained when the same antibodies were used in radioimmunoassay.


Other references

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