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Fast reannealing sequences of dna in allium cepa characterization and chromosomal localization


, : Fast reannealing sequences of dna in allium cepa characterization and chromosomal localization. Cytobios 44(179): 263-272

Fast reannealing sequences of DNA in Allium cepa were localized on metaphase chromosomes and characterized physicochemically. The arrangement of these sequences supported the hypothesis of a prevalent localization of highly repetitive DNA in the telomeric regions in monocotyledons. The melting curve and its first derivative demonstrated the heterogeneous nature of the repetitive fraction. Digestion of total DNA with Eco RI, Bam HI and Hind III restriction endonucleases and hybridization with a 32P-labelled highly repetitive fraction strongly support this view. The repetitive fraction was also characterized by a high content of 5-methylcytosine as indicated by the electrophoretic pattern of Hpa II digests.

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