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Freeze induced phase transitions in the plasma membrane of isolated protoplasts

, : Freeze induced phase transitions in the plasma membrane of isolated protoplasts. Physiologia Plantarum 69(4): 666-674

Protoplasts were enzymically isolated from 2-week-old non-acclimated rye (Secale cereale L. cv. Puma) seedlings. They were resuspended in isotonic sorbitol with different concentrations (0-10%) of dimethyl sulfoxide (DMSO). The survival of the protoplasts frozen in isotonic sorbitol solutions declined at temperatures below the freezing point with the LT50 being C. Addition of DMSO to the osmoticum increased survival at freezing temperatures. The optimum concentration of DMSO was 4% and lowered the LT50 to C. Freeze-fracture studies of the plasma membrane revealed a particulate lipid lamellae at C, but the first appearance of lateral phase separations, striations, and inverted cylindrical micelles (hexagonalII-type structures) occurred at C. At lower temperatures, -8 and C, the occurrence of nonbilayer structures became more common. The addition of DMSO decreased the incidence of the ultrastructural changes. With 2 or 4% DMSO, non-bilayer structures were not observed at temperatures above C. Instead, striations and HII-type structures were observed at -15 and C.


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Daly, F.F.S.; Sidney, K.L.; Fatovich, D.M., 1998: The Medical Emergency Team (MET): A model for the district general hospital. Background: Most hospitals have a Cardiac Arrest Team, activated after cardiopulmonary arrest. The Medical Emergency Team (MET) is a newer concept, encompassing a proactive response to a wide range of emergencies with the aim of preventing irrever...

Hecker, H., 1970: Ultrastructure of symbionts in oocytes of Ornithodorus moubata, Murray (Ixodoidea: Argasidae) following simultaneous glutaraldehyde-osmium fixation and subsequent treatment with uranyl acetate (triple fixation). Experientia 26(8): 874-877

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Casetta, C.; Negretti, F.; Trabattoni, C., 1992: Microbiological control of pharmaceutical products containing preservatives: drawbacks of the method of membrane filtration. Microbiological assays carried out on membranes employed for the filtration of solutions of preservatives with concentration used in pharmaceuticals, demonstrated that such membranes, even after washing with 300 ml (100 ml X 3) of peptone water ac...

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Rey L., 1975: Introduction some basic facts about freeze drying. Goldblith, S A , L Rey And W W Rothmayr (Ed ) Food Science And Technology Freeze Drying And Advanced Food Technology Conference, Buergenstock, Switzerland, June, 1973 Xxxi+730p Illus Academic Press: London, England; New York, N Y , U S A Isbn 0-12-288450-7 Xiii-Xix

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Toda, H.; Abe, T.; Haibara, K., 1997: Carbon mineralization kinetics in forest soil. Characteristics of carbon (C) mineralization in forest soils were investigated in stands of sugi (Japanese cedar, Cryptomeria japonica D. DON) and hinoki (Japanese cypress, Chamaecyparis obtusa ENDL) in east Gunma, and stands of sudajii (Castanops...

Perdrup, A., 1959: Skin and every day products of the chemical industry. Allergic and toxic reactions. Detergents. Maanedsskrift for Praktisk Laegegerning Og Social Medicin 37: 321-336

Amici, G.; Boccaletto, F.; Carniato, A.; Zoli, P.; D.R.n, G.; Teodori, T.; Bocci, C.; Calconi, G.; Palermo, F., 1996: Plasma, peritoneal and urinary clearances with 125I-iothalamate in peritoneal dialysis. Nephrology Dialysis Transplantation 11(6): A249